Trigonella corniculata (L.)L的染色体尺度基因组组装。Nagauri pan /Kasuri methi),一种重要的香料。

IF 6.9 2区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
Ambika Baldev Gaikwad, Sheel Yadav, Ratna Kumari, Wanchha Maurya, Parimalan Rangan, Rakesh Singh, Gyanendra Pratap Singh
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引用次数: 0

摘要

Trigonella corniculata (L) L.或Nagauri pan /Kasuri methi,是一种重要的香料作物,具有很高的营养保健潜力。我们使用高覆盖率PacBio, Illumina和Hi-C reads报道了T. corniculata基因组的从头染色体尺度组装。该组合全长798 Mb (Megabases),包含282个支架,其中一个支架N50为99.6 Mb。超过98%的序列长度被捕获在8个不同的假分子中,平均长度为98 Mb。BUSCO评分超过97%表明基因组具有高度的完整性和连续性。共预测64,801个蛋白质编码基因。全基因组简单序列重复(99,149)已被鉴定并在48个位点进行湿实验室验证。本研究获得的胡芦巴染色体尺度基因组组装和SSR标记将为胡芦巴及其他胡芦巴物种的结构和功能基因组学研究奠定坚实的基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Chromosome-scale genome assembly of Trigonella corniculata (L.)L. (Nagauri pan /Kasuri methi), an important spice.

Chromosome-scale genome assembly of Trigonella corniculata (L.)L. (Nagauri pan /Kasuri methi), an important spice.

Chromosome-scale genome assembly of Trigonella corniculata (L.)L. (Nagauri pan /Kasuri methi), an important spice.

Chromosome-scale genome assembly of Trigonella corniculata (L.)L. (Nagauri pan /Kasuri methi), an important spice.

Trigonella corniculata (L) L. or Nagauri pan /Kasuri methi, is an important spice crop with high nutraceutical potential. We report the de novo chromosome-scale assembly of T. corniculata genome using high coverage PacBio, Illumina and Hi-C reads. The assembly spans 798 Mb (Megabases) in 282 scaffolds with a scaffold N50 of 99.6 Mb. More than 98% of the sequence length is captured in eight different pseudomolecules with an average length of 98 Mb. A BUSCO score of over 97% is suggestive of the high degree of completeness and contiguity of the genome. A total of 64,801 protein-coding genes are predicted. Genome-wide Simple Sequence Repeats (99,149) have been identified and wet lab validated at forty-eight loci. The chromosome-scale genome assembly of T. corniculata and the SSR markers identified in this study will provide a strong foundation for future structural and functional genomics studies in T. corniculata and other fenugreek species.

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来源期刊
Scientific Data
Scientific Data Social Sciences-Education
CiteScore
11.20
自引率
4.10%
发文量
689
审稿时长
16 weeks
期刊介绍: Scientific Data is an open-access journal focused on data, publishing descriptions of research datasets and articles on data sharing across natural sciences, medicine, engineering, and social sciences. Its goal is to enhance the sharing and reuse of scientific data, encourage broader data sharing, and acknowledge those who share their data. The journal primarily publishes Data Descriptors, which offer detailed descriptions of research datasets, including data collection methods and technical analyses validating data quality. These descriptors aim to facilitate data reuse rather than testing hypotheses or presenting new interpretations, methods, or in-depth analyses.
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