在50hz交流设备附近培养辣根过氧化物酶促进其分解和酶活性。

IF 3 3区 工程技术 Q2 CHEMISTRY, ANALYTICAL
Micromachines Pub Date : 2025-03-19 DOI:10.3390/mi16030344
Yuri D Ivanov, Ivan D Shumov, Andrey F Kozlov, Alexander N Ableev, Angelina V Vinogradova, Ekaterina D Nevedrova, Oleg N Afonin, Dmitry D Zhdanov, Vadim Y Tatur, Andrei A Lukyanitsa, Nina D Ivanova, Evgeniy S Yushkov, Dmitry V Enikeev, Vladimir A Konev, Vadim S Ziborov
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引用次数: 0

摘要

众所周知,由变压器等交流电设备产生的低频电磁场会影响酶的物理化学性质和功能,包括它们的催化活性。本文采用原子力显微镜(AFM)和分光光度法研究了50 Hz交流自变压器附近的培养如何影响辣根过氧化物酶(HRP)的理化性质。我们发现,将酶在负载的自变压器线圈上方孵育半小时,促进了HRP单体形式在云母上的吸附,与对照样品相比,吸附酶颗粒的数量增加了两个数量级。最有趣的是,将HRP置于切断电源的变压器上孵育同样一段时间,也发现会导致酶的分解。值得注意的是,在两种情况下都观察到HRP对ABTS的活性增加。我们希望报告的有趣效应将强调在设计用于酶系统操作的实验室和工业设备时考虑低频电磁场对酶的影响的重要性。研究结果表明,为避免交流变压器产生的低频电磁场对人体的不良影响,对交流变压器进行适当的屏蔽是非常重要的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Incubation of Horseradish Peroxidase near 50 Hz AC Equipment Promotes Its Disaggregation and Enzymatic Activity.

Low-frequency electromagnetic fields, induced by alternating current (AC)-based equipment such as transformers, are known to influence the physicochemical properties and function of enzymes, including their catalytic activity. Herein, we have investigated how incubation near a 50 Hz AC autotransformer influences the physicochemical properties of horseradish peroxidase (HRP), by atomic force microscopy (AFM) and spectrophotometry. We found that a half-hour-long incubation of the enzyme above the coil of a loaded autotransformer promoted the adsorption of the monomeric form of HRP on mica, enhancing the number of adsorbed enzyme particles by two orders of magnitude in comparison with the control sample. Most interestingly, the incubation of HRP above the switched-off transformer, which was unplugged from the mains power supply, for the same period of time was also found to cause a disaggregation of the enzyme. Notably, an increase in the activity of HRP against ABTS was observed in both cases. We hope that the interesting effects reported will emphasize the importance of consideration of the influence of low-frequency electromagnetic fields on enzymes in the design of laboratory and industrial equipment intended for operation with enzyme systems. The effects revealed in our study indicate the importance of proper shielding of AC-based transformers in order to avoid the undesirable influence of low-frequency electromagnetic fields induced by these transformers on humans.

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来源期刊
Micromachines
Micromachines NANOSCIENCE & NANOTECHNOLOGY-INSTRUMENTS & INSTRUMENTATION
CiteScore
5.20
自引率
14.70%
发文量
1862
审稿时长
16.31 days
期刊介绍: Micromachines (ISSN 2072-666X) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to micro-scaled machines and micromachinery. It publishes reviews, regular research papers and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.
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