高强度聚焦超声对牙根牙本质内细菌减少、化学结构和力学性能的影响。

IF 1.6 Q3 DENTISTRY, ORAL SURGERY & MEDICINE
Sheetal Maria Rajan, Barsha Shrestha, Mostafa M A Elkholy, Hany Mohamed Aly Ahmed, Amr Fawzy
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引用次数: 0

摘要

目的:高强度聚焦超声(HIFU)在根治细菌方面具有显著的效果,是根管消毒的一种有前景的解决方案。本体外研究探讨了HIFU对减少管内细菌的作用及其对牙根本质化学和力学性质的影响。它还旨在证明声波在根管系统(RCS)内的穿透和分布,并表征相关的温度变化。方法:选取感染粪肠球菌(E. faecalis)并培养2周的单管前磨牙82颗,随机分为阴性对照组、4%次氯酸钠(NaOCl)组、60 s HIFU组和120 s HIFU组(250 kHz/20W)。处理后,从根管中收集生物膜样品,使用集落形成单位(CFU)和3-[4,5-二甲基噻唑-2-基]-2,5二苯基溴化四氮唑(MTT)测定法评估活菌细胞。随后使用扫描电子显微镜、共聚焦激光扫描显微镜(CLSM)、显微硬度和拉曼光谱检查根管表面。利用CLSM和热像仪检测了声波在RCS内的穿透、分布和温度变化。结果:与4% NaOCl相比,60 s和120 s HIFU对粪肠球菌生物膜的抗菌效果一致。MTT和CFU试验显示生物膜活力显著降低,特别是在HIFU暴露120 s时(结论:这种微创方法有望去除RCS中的细菌生物膜,使HIFU成为加强根管消毒的有价值的辅助治疗方法。(eej - 2024 - 08 - 123)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Effect of High-intensity Focused Ultrasound on Intracanal Bacterial Reduction, Chemical Structure, and Mechanical Properties of Root Dentine.

Objective: High-intensity focused ultrasound (HIFU) has demonstrated significant efficacy in eradicating bacteria from substrates emerging as a promising solution for root canal disinfection. This in-vitro study investigated the effects of HIFU on reducing intracanal bacteria and its impact on the chemical and mechanical properties of root dentine. It also aimed to demonstrate acoustic wave penetration and distribution within the root canal system (RCS) and characterize the associated temperature changes.

Methods: Eighty-two extracted premolar teeth with single canals, infected with Enterococcus faecalis (E. faecalis) and cultured for two weeks, were randomly assigned to four groups: negative control, 4% sodium hypochlorite (NaOCl), 60 s HIFU, and 120 s HIFU (operated at 250 kHz/20W). Post-treatment, biofilm samples were collected from the root canals to assess viable bacterial cells using colony-forming unit (CFU) and 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assays. The root canal surfaces were subsequently examined using scanning electron microscopy, confocal laser scanning microscopy (CLSM), microhardness, and Raman spectroscopy. Acoustic wave penetration, distribution, and temperature changes within the RCS were examined using CLSM and thermal camera.

Results: HIFU at 60 s and 120 s consistently demonstrated superior anti-bacterial efficacy against E. faecalis biofilms compared to 4% NaOCl. MTT and CFU assays revealed a significant reduction in biofilm viability, particularly at 120 s of HIFU exposure (p<0.05). CLSM and SEM analyses demonstrated enhanced penetration and detachment of biofilms, as well as improved smear layer removal and preservation of dentinal tubules, especially at 120 s of HIFU exposure. HIFU treatment did not adversely affect the amide/mineral content of root dentine or its surface microhardness. Additionally, HIFU enhanced acoustic wave propagation and resulted in a controlled increase in temperature within the root canal over time.

Conclusion: This minimally invasive approach shows promise for removing bacterial biofilms in the RCS, positioning HIFU as a valuable adjunctive treatment for enhancing root canal disinfection. (EEJ-2024-08-123).

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来源期刊
European Endodontic Journal
European Endodontic Journal DENTISTRY, ORAL SURGERY & MEDICINE-
CiteScore
3.40
自引率
5.60%
发文量
25
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