Taklimakanibacter albus sp. nov., an Endophytic Bacterial Species Isolated from the Root of Paris polyphylla var. yunnanensis.

A Gram-negative, white-pigment, rod-shaped bacterial strain YIM B02566^T was isolated from the root of Paris polyphylla var. yunnanensis in China. Strain YIM B02566^T exhibited optimal growth at 28 °C and pH 7.0 on Reasoner's 2A agar without NaCl supplementation. Phylogenetic analyses based on the 16S rRNA gene sequences and the concatenation of 433 single-copy orthologous genes revealed that strain YIM B02566^T formed a distinct lineage in the genus Taklimakanibacter. The sequence similarity of the 16S rRNA gene between YIM B02566^T and two known species of genus Taklimakanibacter were 99.12% (T. deserti SYSU D60010^T) and 99.05% (T. lacteus SYSU D60012^T), respectively. The principal fatty acids were C_19:0 cyclo ω8c, C_16:0, and summed feature 8. Strain YIM B02566^T contained UQ-10 as the sole respiratory quinone. The polar lipids of the strain comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified phospholipid, two unidentified aminolipids, and an unidentified polar lipid. The whole-genome analysis indicated that the genome size was 7.2 Mbp, and the genomic DNA G + C content was 62.9%. The digital DNA-DNA hybridization values between strain YIM B02566^T and two Taklimakanibacter species were 24.50% (T. deserti SYSU D60010^T) and 25.40% (T. lacteus SYSU D60012^T). Meanwhile, the ANI and AAI values between strain YIM B02566^T and its closest neighbours are well below the proposed thresholds for species determination. Therefore, based on polyphasic analysis, the isolate represents a novel species, for which the name Taklimakanibacter albus is proposed. The type strain is YIM B02566^T (= CGMCC 1.18656^T = JCM 34643^T).