姜黄素和阿霉素对视网膜肿瘤细胞凋亡的协同作用:分子和细胞分析。

IF 1.2 4区 医学 Q3 ANATOMY & MORPHOLOGY
Semih Doğan, Mehmet Cudi Tuncer, İlhan Özdemir
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引用次数: 0

摘要

视网膜母细胞瘤(Retinoblastoma, RB)是一种高度侵袭性的眼部肿瘤,由于社会经济和医疗条件的限制,它直接影响到许多儿童。虽然治疗RB的方法多种多样,但并不能得到满意的结果。植物源性天然产物在治疗各种肿瘤方面已显示出明确的治疗效果,被广泛应用于RB的研究。姜黄素(Curcumin, Cur)与多种调节蛋白的表达和活性密切相关。在这项研究中,姜黄素联合强化疗药物如阿霉素(Adr)的抗癌作用被证实。材料与方法:采用人视网膜母细胞瘤细胞系WERI-Rb-1 (HTB-169)。经测定,在IC50剂量的Cur和Adr联合应用48小时后,癌细胞的细胞毒性增加。通过定量同步PCR (qRT-PCR)检测Caspase-3、P53和Survivin基因mRNA水平的差异,这些基因是处理细胞凋亡的重要触发和调节因子。结果:应用Cur和Adr导致Caspase-3和P53 mRNA水平显著升高,Survivin mRNA水平显著降低(p < 0.05)。本研究首次发现Cur和Adr可通过触发视网膜母细胞瘤细胞系的凋亡死亡途径发挥抗癌作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Synergistic effect of curcumin and adriamycin on apoptosis in retinal tumor cells: a molecular and cellular analysis.

Introduction: Retinoblastoma (RB) is a highly aggressive eye tumor and directly affects many children due to socioeconomic and medical constraints. Although there are various approaches to the treatment of RB, satisfactory results cannot be obtained. Natural products of plant origin have shown definite therapeutic effects in the treatment of various tumors and are widely used in the study of RB. Curcumin (Cur) is closely related to the expression and activity of various regulatory proteins. In this study, the anticancer roles of the combination of curcumin with a strong chemotherapy agent such as adryamycin (Adr) were demonstrated.

Materials and methods: The human retinoblastoma cell line WERI-Rb-1 (HTB-169) was used in the study. It was determined that cytotoxicity increased in cancer cells treated with IC50 doses of Cur and Adr for 48 hours in combined applications. Differences in mRNA levels of Caspase-3, P53 and Survivin genes, which are important triggers and regulators of apoptosis in treated cells, were determined by quantitative simultaneous PCR (qRT-PCR).

Results: While it was determined that Cur and Adr application caused a significant increase in both Caspase-3 and P53 mRNA levels, a significant decrease was detected in the mRNA level of Survivin (p < 0.05). In this study, it was shown for the first time that Cur and Adr can exhibit anti-cancer properties by triggering the apoptosis death pathway in retinoblastoma cell lines.

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来源期刊
Folia morphologica
Folia morphologica ANATOMY & MORPHOLOGY-
CiteScore
2.40
自引率
0.00%
发文量
218
审稿时长
6-12 weeks
期刊介绍: "Folia Morphologica" is an official journal of the Polish Anatomical Society (a Constituent Member of European Federation for Experimental Morphology - EFEM). It contains original articles and reviews on morphology in the broadest sense (descriptive, experimental, and methodological). Papers dealing with practical application of morphological research to clinical problems may also be considered. Full-length papers as well as short research notes can be submitted. Descriptive papers dealing with non-mammals, cannot be accepted for publication with some exception.
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