人巨噬细胞条件培养基对TGF-β诱导的肺成纤维细胞分化的影响

IF 0.9 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Bulletin of Experimental Biology and Medicine Pub Date : 2025-02-01 Epub Date: 2025-03-26 DOI:10.1007/s10517-025-06353-7

A A Maksimova, E Ya Shevela, M A Tikhonova, I M Rashchupkin, A A Ostanin, E R Chernykh

{"title":"人巨噬细胞条件培养基对TGF-β诱导的肺成纤维细胞分化的影响","authors":"A A Maksimova, E Ya Shevela, M A Tikhonova, I M Rashchupkin, A A Ostanin, E R Chernykh","doi":"10.1007/s10517-025-06353-7","DOIUrl":null,"url":null,"abstract":"We studied the ability of human macrophage conditioned media to inhibit differentiation of lung fibroblasts in vitro. The results showed that the conditioned medium of M-CSF-differentiated non-polarized macrophages (induced by macrophage CSF) suppressed TGF-β-induced differentiation, reduced the expression of myofibroblast markers (α-smooth muscle actin and vimentin), and decreased the contractile activity of lung myofibroblasts, while the conditioned media of dexamethasone-polarized macrophages and GM-CSF-differentiated non-polarized macrophages (differentiation stimulus is granulocyte-macrophage CSF) produced no such effects. Thus, the obtained data indicate the antifibrogenic potential of non-polarized M-CSF-differentiated macrophages.","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":" ","pages":"442-446"},"PeriodicalIF":0.9000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of Human Macrophage Conditioned Media on TGF-β-Induced Differentiation of Lung Fibroblasts.\",\"authors\":\"A A Maksimova, E Ya Shevela, M A Tikhonova, I M Rashchupkin, A A Ostanin, E R Chernykh\",\"doi\":\"10.1007/s10517-025-06353-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We studied the ability of human macrophage conditioned media to inhibit differentiation of lung fibroblasts in vitro. The results showed that the conditioned medium of M-CSF-differentiated non-polarized macrophages (induced by macrophage CSF) suppressed TGF-β-induced differentiation, reduced the expression of myofibroblast markers (α-smooth muscle actin and vimentin), and decreased the contractile activity of lung myofibroblasts, while the conditioned media of dexamethasone-polarized macrophages and GM-CSF-differentiated non-polarized macrophages (differentiation stimulus is granulocyte-macrophage CSF) produced no such effects. Thus, the obtained data indicate the antifibrogenic potential of non-polarized M-CSF-differentiated macrophages.\",\"PeriodicalId\":9331,\"journal\":{\"name\":\"Bulletin of Experimental Biology and Medicine\",\"volume\":\" \",\"pages\":\"442-446\"},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2025-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bulletin of Experimental Biology and Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s10517-025-06353-7\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/3/26 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"MEDICINE, RESEARCH & EXPERIMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of Experimental Biology and Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s10517-025-06353-7","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/3/26 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}

引用次数: 0

摘要

我们在体外研究了人巨噬细胞条件培养基对肺成纤维细胞分化的抑制作用。结果表明，m -CSF诱导的非极化巨噬细胞条件培养基抑制TGF-β诱导的分化，降低肌成纤维细胞标志物（α-平滑肌肌动蛋白和波形蛋白）的表达，降低肺肌成纤维细胞的收缩活性；而地塞米松极化巨噬细胞和gm -CSF分化的非极化巨噬细胞（分化刺激为粒细胞-巨噬细胞CSF）的条件培养基则没有这种作用。因此，获得的数据表明非极化m - csf分化的巨噬细胞具有抗纤维化的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Effect of Human Macrophage Conditioned Media on TGF-β-Induced Differentiation of Lung Fibroblasts.

We studied the ability of human macrophage conditioned media to inhibit differentiation of lung fibroblasts in vitro. The results showed that the conditioned medium of M-CSF-differentiated non-polarized macrophages (induced by macrophage CSF) suppressed TGF-β-induced differentiation, reduced the expression of myofibroblast markers (α-smooth muscle actin and vimentin), and decreased the contractile activity of lung myofibroblasts, while the conditioned media of dexamethasone-polarized macrophages and GM-CSF-differentiated non-polarized macrophages (differentiation stimulus is granulocyte-macrophage CSF) produced no such effects. Thus, the obtained data indicate the antifibrogenic potential of non-polarized M-CSF-differentiated macrophages.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.