{"title":"甲氧基聚乙二醇丙酸聚合物的高通量绿色超高性能收敛色谱-串联质谱分析方法的建立与验证。","authors":"Chunpeng Feng, Jiye Tian, Qisheng Fang, Yajie Cheng, Yue Deng, JiaRui Zhang, Shuang Feng, Qingbin Wang, Hecheng Wang, Xuan Zhao, Lei Yin","doi":"10.1007/s00216-025-05849-5","DOIUrl":null,"url":null,"abstract":"<p><p>As a synthetic polymer, methoxy-polyethylene glycol propionic acid (M-PEG-PA) is widely used in the biomedicine field. Unraveling the pharmacokinetic behavior of M-PEG<sub>6</sub>-PA in vivo is crucial for evaluating the safety and efficiency of M-PEG-PA-related polymers or drug delivery systems. A high-throughput and green ultra-performance convergence chromatography-tandem mass spectrometry (UPC<sup>2</sup>-MS/MS) assay was firstly developed and validated for the determination of M-PEG<sub>6</sub>-PA polymers in a biological matrix. The MRM transition (mass to charge ratio, precursor ions→fragment ions) of m/z 367.2→118.9 was used to quantify M-PEG<sub>6</sub>-PA in this study. The throughput of the assay is high and the total running time for each sample was only 2 min. The linear range of the developed UPC<sup>2</sup>-MS/MS assay for quantification of M-PEG<sub>6</sub>-PA in a biological matrix is 0.05 to 30 μg/mL (R≥0.995). Intra-day and inter-day precisions for the determination of M-PEG<sub>6</sub>-PA by this analytical assay were <6.99%. The absolute recoveries and matrix effects of M-PEG<sub>6</sub>-PA ranged from 79.50 to 92.47% and 68.72 to 81.73%, respectively. The UPC<sup>2</sup>-MS/MS assay was successfully applied to quantify the concentrations of M-PEG<sub>6</sub>-PA polymers in rat plasma samples.</p>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":" ","pages":""},"PeriodicalIF":3.8000,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Development and validation of a high-throughput and green ultra-performance convergence chromatography-tandem mass spectrometry assay for quantification of methoxy-polyethylene glycol propionic acid polymers.\",\"authors\":\"Chunpeng Feng, Jiye Tian, Qisheng Fang, Yajie Cheng, Yue Deng, JiaRui Zhang, Shuang Feng, Qingbin Wang, Hecheng Wang, Xuan Zhao, Lei Yin\",\"doi\":\"10.1007/s00216-025-05849-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>As a synthetic polymer, methoxy-polyethylene glycol propionic acid (M-PEG-PA) is widely used in the biomedicine field. Unraveling the pharmacokinetic behavior of M-PEG<sub>6</sub>-PA in vivo is crucial for evaluating the safety and efficiency of M-PEG-PA-related polymers or drug delivery systems. A high-throughput and green ultra-performance convergence chromatography-tandem mass spectrometry (UPC<sup>2</sup>-MS/MS) assay was firstly developed and validated for the determination of M-PEG<sub>6</sub>-PA polymers in a biological matrix. The MRM transition (mass to charge ratio, precursor ions→fragment ions) of m/z 367.2→118.9 was used to quantify M-PEG<sub>6</sub>-PA in this study. The throughput of the assay is high and the total running time for each sample was only 2 min. The linear range of the developed UPC<sup>2</sup>-MS/MS assay for quantification of M-PEG<sub>6</sub>-PA in a biological matrix is 0.05 to 30 μg/mL (R≥0.995). Intra-day and inter-day precisions for the determination of M-PEG<sub>6</sub>-PA by this analytical assay were <6.99%. The absolute recoveries and matrix effects of M-PEG<sub>6</sub>-PA ranged from 79.50 to 92.47% and 68.72 to 81.73%, respectively. The UPC<sup>2</sup>-MS/MS assay was successfully applied to quantify the concentrations of M-PEG<sub>6</sub>-PA polymers in rat plasma samples.</p>\",\"PeriodicalId\":462,\"journal\":{\"name\":\"Analytical and Bioanalytical Chemistry\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2025-03-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical and Bioanalytical Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1007/s00216-025-05849-5\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical and Bioanalytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s00216-025-05849-5","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Development and validation of a high-throughput and green ultra-performance convergence chromatography-tandem mass spectrometry assay for quantification of methoxy-polyethylene glycol propionic acid polymers.
As a synthetic polymer, methoxy-polyethylene glycol propionic acid (M-PEG-PA) is widely used in the biomedicine field. Unraveling the pharmacokinetic behavior of M-PEG6-PA in vivo is crucial for evaluating the safety and efficiency of M-PEG-PA-related polymers or drug delivery systems. A high-throughput and green ultra-performance convergence chromatography-tandem mass spectrometry (UPC2-MS/MS) assay was firstly developed and validated for the determination of M-PEG6-PA polymers in a biological matrix. The MRM transition (mass to charge ratio, precursor ions→fragment ions) of m/z 367.2→118.9 was used to quantify M-PEG6-PA in this study. The throughput of the assay is high and the total running time for each sample was only 2 min. The linear range of the developed UPC2-MS/MS assay for quantification of M-PEG6-PA in a biological matrix is 0.05 to 30 μg/mL (R≥0.995). Intra-day and inter-day precisions for the determination of M-PEG6-PA by this analytical assay were <6.99%. The absolute recoveries and matrix effects of M-PEG6-PA ranged from 79.50 to 92.47% and 68.72 to 81.73%, respectively. The UPC2-MS/MS assay was successfully applied to quantify the concentrations of M-PEG6-PA polymers in rat plasma samples.
期刊介绍：
Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
