{"title":"基于多孔碳Z-8封装鲁米诺的微滴增强化学发光检测恩诺沙星策略","authors":"Wanfeng Zhang, Liyuan Yu, Liangyu Chen, Xu Hun","doi":"10.1002/bio.70145","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>A droplet-enhanced chemiluminescence (CL) assay for enrofloxacin was fabricated. After preparation ZIF-8 carbonization, porous carbon material Z-800 was obtained and aptamer and cDNA were immobilizated onto the surface of Z-800. Luminol was encapsulated into Z-800 with the help of aptamer and cDNA. In the presence of enrofloxacin, aptamer accurately recognizes it, enrofloxacin specifically binds to aptamer, and the switch that encapsulateed luminol is turned on, causing luminol to be released into the supernatant. The supernatant reacts with H<sub>2</sub>O<sub>2</sub> to produce a strong CL signal. The immiscible organic droplets, benzyl benzoate (Bnbzo), greatly enhances the CL signal. This assay shows a linear relationship between enhanced CL signals and enrofloxacin concentrations in the range from 0.1 to 500.0 nM with LOD of 30 pM (S/N = 3). The sample measurement was performed with the recoveries of 92.00% to 95.63% and relative standard deviation of 3.9%–5.2%. It proves that droplet-enhanced CL is a promising method for enhancing CL signals in real sample and trace analysis.</p>\n </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"40 4","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Droplet-Enhanced Chemiluminescence Strategy for Enrofloxacin Detection Based on Porous Carbon Z-8 Encapsulated Luminol\",\"authors\":\"Wanfeng Zhang, Liyuan Yu, Liangyu Chen, Xu Hun\",\"doi\":\"10.1002/bio.70145\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>A droplet-enhanced chemiluminescence (CL) assay for enrofloxacin was fabricated. After preparation ZIF-8 carbonization, porous carbon material Z-800 was obtained and aptamer and cDNA were immobilizated onto the surface of Z-800. Luminol was encapsulated into Z-800 with the help of aptamer and cDNA. In the presence of enrofloxacin, aptamer accurately recognizes it, enrofloxacin specifically binds to aptamer, and the switch that encapsulateed luminol is turned on, causing luminol to be released into the supernatant. The supernatant reacts with H<sub>2</sub>O<sub>2</sub> to produce a strong CL signal. The immiscible organic droplets, benzyl benzoate (Bnbzo), greatly enhances the CL signal. This assay shows a linear relationship between enhanced CL signals and enrofloxacin concentrations in the range from 0.1 to 500.0 nM with LOD of 30 pM (S/N = 3). The sample measurement was performed with the recoveries of 92.00% to 95.63% and relative standard deviation of 3.9%–5.2%. It proves that droplet-enhanced CL is a promising method for enhancing CL signals in real sample and trace analysis.</p>\\n </div>\",\"PeriodicalId\":49902,\"journal\":{\"name\":\"Luminescence\",\"volume\":\"40 4\",\"pages\":\"\"},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2025-03-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Luminescence\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/bio.70145\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Luminescence","FirstCategoryId":"92","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/bio.70145","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Droplet-Enhanced Chemiluminescence Strategy for Enrofloxacin Detection Based on Porous Carbon Z-8 Encapsulated Luminol
A droplet-enhanced chemiluminescence (CL) assay for enrofloxacin was fabricated. After preparation ZIF-8 carbonization, porous carbon material Z-800 was obtained and aptamer and cDNA were immobilizated onto the surface of Z-800. Luminol was encapsulated into Z-800 with the help of aptamer and cDNA. In the presence of enrofloxacin, aptamer accurately recognizes it, enrofloxacin specifically binds to aptamer, and the switch that encapsulateed luminol is turned on, causing luminol to be released into the supernatant. The supernatant reacts with H2O2 to produce a strong CL signal. The immiscible organic droplets, benzyl benzoate (Bnbzo), greatly enhances the CL signal. This assay shows a linear relationship between enhanced CL signals and enrofloxacin concentrations in the range from 0.1 to 500.0 nM with LOD of 30 pM (S/N = 3). The sample measurement was performed with the recoveries of 92.00% to 95.63% and relative standard deviation of 3.9%–5.2%. It proves that droplet-enhanced CL is a promising method for enhancing CL signals in real sample and trace analysis.
期刊介绍：
Luminescence provides a forum for the publication of original scientific papers, short communications, technical notes and reviews on fundamental and applied aspects of all forms of luminescence, including bioluminescence, chemiluminescence, electrochemiluminescence, sonoluminescence, triboluminescence, fluorescence, time-resolved fluorescence and phosphorescence. Luminescence publishes papers on assays and analytical methods, instrumentation, mechanistic and synthetic studies, basic biology and chemistry.
Luminescence also publishes details of forthcoming meetings, information on new products, and book reviews. A special feature of the Journal is surveys of the recent literature on selected topics in luminescence.
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