基于鲁米诺功能化PPy/AgNPs纳米复合材料的新型口腔癌标志物监测无标记电化学发光免疫传感器

IF 6.8 2区工程技术 Q1 ENGINEERING, MULTIDISCIPLINARY

alexandria engineering journal Pub Date : 2025-03-28 DOI:10.1016/j.aej.2025.03.093

Songlin Zhou , Yue Li , Peiru Yang , Yutao Qin , Anwen Lei , Chao Deng , Yi Sun , Jue Zhang , Hai Liu

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引用次数: 0

摘要

本研究旨在开发一种先进的无标记电化学发光（ECL）免疫传感器，用于选择性和敏感地检测作为口腔癌生物标志物的白细胞介素-8 （IL-8）抗原。ECL免疫传感器依赖于抗il -8抗体偶联到鲁米诺功能化聚吡咯-银纳米颗粒（PPy/AgNPs）纳米复合材料。PPy/AgNPs纳米复合材料促进了抗体和鲁米诺形成的ECL信号的固定化。该免疫传感器具有低LOD （3.9 fg/mL）、宽线性范围（10−3 ~ 103 pg/mL）、出色的特异性、显著的稳定性和可重复性。结果表明，加标回收率为92.00 % ~ 99.30 %，相对标准偏差为3.62 % ~ 4.22 %，适用于加标后的人唾液和血清样品，证实了该免疫传感器在复杂生物体液中的性能和效率。IL-8免疫传感器具有良好的选择性和敏感性，加上其稳定性、低成本和易用性，在早期口腔癌诊断和疾病监测方面具有巨大的潜力。

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A novel label-free electrochemiluminescence immunosensor for oral cancer marker monitoring based on luminol-functionalized PPy/AgNPs nanocomposite

This study was performed to develop a developed label-free electrochemiluminescence (ECL) immunosensor for the selective and sensitive detection of interleukin-8 (IL-8) antigen as a biomarker in oral cancer. The ECL immunosensor relied on an anti-IL-8 antibody conjugated to a luminol-functionalized polypyrrole-silver nanoparticle (PPy/AgNPs) nanocomposite. The PPy/AgNPs nanocomposite promoted both the immobilization of the antibody and the ECL signal formed by luminol. The immunosensor exhibited a low LOD (3.9 fg/mL), a broad linear range (10⁻³ to 10³ pg/mL), excellent specificity, and significant stability and repeatability. Results showed that the recoveries ranging from 92.00 % to 99.30 % and relative standard deviation from 3.62 % to 4.22 % indicated successful application to spiked human saliva and serum samples, confirming the immunosensor's performance and efficiency in complex biological fluids. The excellent selectivity and sensitivity of the proposed IL-8 immunosensor, coupled with its stability, low cost and ease of use, exhibited its substantial potential for early oral cancer diagnosis and disease monitoring.

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来源期刊

alexandria engineering journal Engineering-General Engineering

CiteScore

11.20

自引率

4.40%

发文量

1015

审稿时长

43 days

期刊介绍： Alexandria Engineering Journal is an international journal devoted to publishing high quality papers in the field of engineering and applied science. Alexandria Engineering Journal is cited in the Engineering Information Services (EIS) and the Chemical Abstracts (CA). The papers published in Alexandria Engineering Journal are grouped into five sections, according to the following classification: • Mechanical, Production, Marine and Textile Engineering • Electrical Engineering, Computer Science and Nuclear Engineering • Civil and Architecture Engineering • Chemical Engineering and Applied Sciences • Environmental Engineering