拮抗乳酸菌对大肠杆菌O157:H7生物膜的生理、微观和蛋白质组学性能研究

IF 5 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

International journal of food microbiology Pub Date : 2025-03-24 DOI:10.1016/j.ijfoodmicro.2025.111173

Lucia Cisneros , Ayelen Antonella Baillo , Diego Ploper , María Pia Valacco , Silvia Moreno , Lucila Saavedra , Carlos Minahk , Vincenzina Fusco , Osvaldo Yantorno , Silvina Fadda

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引用次数: 0

摘要

肠出血性大肠杆菌O157:H7 （EHEC）生物膜由于其弹性、持久性和提高病原体在加工表面存活的能力，在食品工业中是一个关键问题。控制这些生物膜对于减轻肠出血性大肠杆菌相关风险至关重要。本研究评估了三种乳酸菌（LAB）菌株——植物乳酸杆菌CRL1075、L. plantarum CRL1482和pentosacpediococcus crl2145——在模拟肉类工业环境（12°C、不锈钢和肉类培养基）下，通过排斥、竞争和置换策略抑制肠出血性大肠杆菌生物膜的潜力。扫描电镜和激光共聚焦扫描显微镜显示肠出血性大肠杆菌生物膜具有较高的粘附性和坚固性。引入LAB菌株可显著减少肠出血性大肠杆菌生物膜的形成，其中竞争策略最为有效，最多可减少6.5 log单位的生物膜。排除和置换策略也降低了病原体的活力，P. pentosaceus CRL2145在所有条件下都表现出一致的抑制效果。EHEC-CRL2145混合生物膜的生物量和覆盖表面积减少，LAB聚集在大肠杆菌细胞周围，限制了它们的生长和粘附。蛋白质组学分析显示，与基本代谢途径、应激反应和生物膜维持相关的85种肠出血性大肠杆菌蛋白受到抑制，这损害了病原体维持生物膜完整性和活力的能力。此外，一些参与粘附和毒力的肠出血性大肠杆菌蛋白被上调，可能是对LAB拮抗的应激反应。重要的是，虽然最近报道了乳酸菌对食源性病原体的抗菌潜力，但这是第一个描述肠出血性大肠杆菌生物膜在乳酸菌攻击时代谢反应的研究。

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Physiological, microscopic and proteomic performance of Escherichia coli O157:H7 biofilms challenged with antagonistic lactic acid bacteria as a bio-decontamination tool for the food industry

Enterohemorrhagic Escherichia coli O157:H7 (EHEC) biofilms are a critical concern in food industry due to their resilience, persistence, and ability to enhance pathogen survival on processing surfaces. Controlling these biofilms is paramount to mitigating the risks associated with EHEC. This work evaluates the potential of three lactic acid bacteria (LAB) strains—Lactiplantibacillus plantarum CRL1075, L. plantarum CRL1482, and Pediococcus pentosaceus CRL2145—as biocontrol agents to inhibit EHEC biofilms through exclusion, competition, and displacement strategies under conditions mimicking meat industry environment (12 °C, stainless steel, and meat-based culture medium). EHEC biofilms showed by scanning electron microscopy and confocal laser scanning microscopy high adherence and robustness. Introducing LAB strains led to substantial reductions in EHEC biofilm formation, with the competition strategy emerging most effective, reducing biofilms by up to 6.5 log units. Exclusion and displacement strategies also decreased pathogen viability, with P. pentosaceus CRL2145 showing consistent inhibitory effects across all conditions. EHEC-CRL2145 mixed biofilms exhibited reduced biomass and covered surface area, with LAB clustered around E. coli cells, limiting their growth and adhesion. Proteomic analysis revealed repression of 85 EHEC proteins associated with essential metabolic pathways, stress responses, and biofilm maintenance, which compromised the pathogen's ability to sustain biofilm integrity and viability. Additionally, some EHEC proteins involved in adhesion and virulence were upregulated, possibly as stress response to LAB antagonism. Importantly, while the antimicrobial potential of LAB against foodborne pathogens was recently reported, this is the first study to describe the metabolic response of EHEC biofilms when challenged by LAB.

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来源期刊

International journal of food microbiology 工程技术-食品科技

CiteScore

10.40

自引率

5.60%

发文量

322

审稿时长

65 days

期刊介绍： The International Journal of Food Microbiology publishes papers dealing with all aspects of food microbiology. Articles must present information that is novel, has high impact and interest, and is of high scientific quality. They should provide scientific or technological advancement in the specific field of interest of the journal and enhance its strong international reputation. Preliminary or confirmatory results as well as contributions not strictly related to food microbiology will not be considered for publication.