微藻衍生的微颗粒通过糖酵解和MAPK活化联合改善免疫调节

IF 3.9 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Langmuir Pub Date : 2025-03-27 DOI:10.1021/acs.langmuir.4c05088

Saetbyeol Jeon, Hwira Baek, Seulgi Kim, Youngseok Kim, Junoh Kim, Jin Woong Kim

{"title":"微藻衍生的微颗粒通过糖酵解和MAPK活化联合改善免疫调节","authors":"Saetbyeol Jeon, Hwira Baek, Seulgi Kim, Youngseok Kim, Junoh Kim, Jin Woong Kim","doi":"10.1021/acs.langmuir.4c05088","DOIUrl":null,"url":null,"abstract":"Natural polysaccharides possess potent immunostimulatory properties, but their poor solubility impedes efficiency of cellular delivery. This study focuses on extraction of microparticles (MPs) fromEuglena gracilis, a microalgae species characterized by abundant intracellular β-1,3-glucan and flexible cell membrane. We introduce anE. gracilis-derived MP (MPEG) system as a natural carrier for solubilized β-glucan. The MPEG system enhances β-glucan’s solubility and loading efficiency through sequential sonication and cell extrusion. In vitro studies reveal that MPEG utilizes multiple endocytosis pathways, including phagocytosis, clathrin-mediated, and lipid raft-mediated routes, for effective β-glucan delivery into cells. Upon cellular internalization, MPEG components trigger dual activation of the MAPK signaling pathway and glycolysis in macrophages, leading to enhanced production of pro-inflammatory cytokines and lactic acid, ultimately strengthening innate immune responses. This MPEG system offers a promising approach to harnessing the immunostimulatory properties of natural polysaccharides while overcoming their solubility limitations, opening new avenues for targeted cellular delivery in immunomodulation therapies.","PeriodicalId":50,"journal":{"name":"Langmuir","volume":"50 1","pages":""},"PeriodicalIF":3.9000,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Microalgae-Derived Microparticles Improve Immunomodulation via Combined Glycolysis and MAPK Activation\",\"authors\":\"Saetbyeol Jeon, Hwira Baek, Seulgi Kim, Youngseok Kim, Junoh Kim, Jin Woong Kim\",\"doi\":\"10.1021/acs.langmuir.4c05088\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Natural polysaccharides possess potent immunostimulatory properties, but their poor solubility impedes efficiency of cellular delivery. This study focuses on extraction of microparticles (MPs) fromEuglena gracilis, a microalgae species characterized by abundant intracellular β-1,3-glucan and flexible cell membrane. We introduce anE. gracilis-derived MP (MPEG) system as a natural carrier for solubilized β-glucan. The MPEG system enhances β-glucan’s solubility and loading efficiency through sequential sonication and cell extrusion. In vitro studies reveal that MPEG utilizes multiple endocytosis pathways, including phagocytosis, clathrin-mediated, and lipid raft-mediated routes, for effective β-glucan delivery into cells. Upon cellular internalization, MPEG components trigger dual activation of the MAPK signaling pathway and glycolysis in macrophages, leading to enhanced production of pro-inflammatory cytokines and lactic acid, ultimately strengthening innate immune responses. This MPEG system offers a promising approach to harnessing the immunostimulatory properties of natural polysaccharides while overcoming their solubility limitations, opening new avenues for targeted cellular delivery in immunomodulation therapies.\",\"PeriodicalId\":50,\"journal\":{\"name\":\"Langmuir\",\"volume\":\"50 1\",\"pages\":\"\"},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2025-03-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Langmuir\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1021/acs.langmuir.4c05088\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Langmuir","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1021/acs.langmuir.4c05088","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

摘要

天然多糖具有有效的免疫刺激特性，但其溶解度差阻碍了细胞递送的效率。摘要本研究主要从微藻中提取微颗粒（MPs），该微藻具有丰富的胞内β-1,3-葡聚糖和柔软的细胞膜。我们介绍anE。细股肌衍生的MP （MPEG）系统作为可溶性β-葡聚糖的天然载体。MPEG系统通过连续超声和细胞挤压提高β-葡聚糖的溶解度和负载效率。体外研究表明，MPEG利用多种内吞途径，包括吞噬、网格蛋白介导和脂质筏介导的途径，将β-葡聚糖有效地递送到细胞中。在细胞内化后，MPEG成分触发巨噬细胞MAPK信号通路和糖酵解的双重激活，导致促炎细胞因子和乳酸的产生增加，最终增强先天免疫反应。这种MPEG系统提供了一种很有前途的方法来利用天然多糖的免疫刺激特性，同时克服其溶解度限制，为免疫调节治疗中的靶向细胞递送开辟了新的途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Microalgae-Derived Microparticles Improve Immunomodulation via Combined Glycolysis and MAPK Activation

查看原文本刊更多论文

Microalgae-Derived Microparticles Improve Immunomodulation via Combined Glycolysis and MAPK Activation

Natural polysaccharides possess potent immunostimulatory properties, but their poor solubility impedes efficiency of cellular delivery. This study focuses on extraction of microparticles (MPs) fromEuglena gracilis, a microalgae species characterized by abundant intracellular β-1,3-glucan and flexible cell membrane. We introduce anE. gracilis-derived MP (MP_EG) system as a natural carrier for solubilized β-glucan. The MP_EG system enhances β-glucan’s solubility and loading efficiency through sequential sonication and cell extrusion. In vitro studies reveal that MP_EG utilizes multiple endocytosis pathways, including phagocytosis, clathrin-mediated, and lipid raft-mediated routes, for effective β-glucan delivery into cells. Upon cellular internalization, MP_EG components trigger dual activation of the MAPK signaling pathway and glycolysis in macrophages, leading to enhanced production of pro-inflammatory cytokines and lactic acid, ultimately strengthening innate immune responses. This MP_EG system offers a promising approach to harnessing the immunostimulatory properties of natural polysaccharides while overcoming their solubility limitations, opening new avenues for targeted cellular delivery in immunomodulation therapies.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Langmuir 化学-材料科学：综合

CiteScore

6.50

自引率

10.30%

发文量

1464

审稿时长

2.1 months

期刊介绍： Langmuir is an interdisciplinary journal publishing articles in the following subject categories: Colloids: surfactants and self-assembly, dispersions, emulsions, foams Interfaces: adsorption, reactions, films, forces Biological Interfaces: biocolloids, biomolecular and biomimetic materials Materials: nano- and mesostructured materials, polymers, gels, liquid crystals Electrochemistry: interfacial charge transfer, charge transport, electrocatalysis, electrokinetic phenomena, bioelectrochemistry Devices and Applications: sensors, fluidics, patterning, catalysis, photonic crystals However, when high-impact, original work is submitted that does not fit within the above categories, decisions to accept or decline such papers will be based on one criteria: What Would Irving Do? Langmuir ranks #2 in citations out of 136 journals in the category of Physical Chemistry with 113,157 total citations. The journal received an Impact Factor of 4.384*. This journal is also indexed in the categories of Materials Science (ranked #1) and Multidisciplinary Chemistry (ranked #5).