单向重排可以获得高度稳定的螺旋环氮氧化物，用于蛋白质自旋标记

IF 4.2 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Chemical Communications Pub Date : 2025-04-09 DOI:10.1039/d5cc00472a

Mateusz P. Sowiński , Elena M. Mocanu , Hannah Ruskin-Dodd , Aidan P. McKay , David B. Cordes , Janet E. Lovett , Marius Haugland-Grange

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引用次数: 0

摘要

自旋标记使使用电子顺磁共振（EPR）光谱研究生物分子成为可能。在这里，我们描述了基于含有碘乙酰胺部分的螺旋环吡咯烷基氮氧化物的半胱氨酸反应性自旋标记的合成。结果表明，自旋标签在还原条件下具有高度的持久性，同时在180 K温度下保持良好的EPR弛豫参数。在成功标记钙调素变体的双自旋后，用EPR实验双电子-电子共振（DEER）在120 K下测量了自旋间距离。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Sigmatropic rearrangement enables access to a highly stable spirocyclic nitroxide for protein spin labelling†

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Sigmatropic rearrangement enables access to a highly stable spirocyclic nitroxide for protein spin labelling†

Spin labelling enables the study of biomolecules using electron paramagnetic resonance (EPR) spectroscopy. Here, we describe the synthesis of a cysteine-reactive spin label based on a spirocyclic pyrrolidinyl nitroxide containing an iodoacetamide moiety. The spin label was shown to be highly persistent under reducing conditions while maintaining excellent EPR relaxation parameters up to a temperature of 180 K. After successful double spin labelling of a calmodulin variant, interspin distances were measured by the EPR experiment double electron–electron resonance (DEER) at 120 K.

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来源期刊

Chemical Communications 化学-化学综合

CiteScore

8.60

自引率

4.10%

发文量

2705

审稿时长

1.4 months

期刊介绍： ChemComm (Chemical Communications) is renowned as the fastest publisher of articles providing information on new avenues of research, drawn from all the world''s major areas of chemical research.