Gabrielle Gomes Centenaro, Michael Willian Favoreto, Taynara de Souza Carneiro, Michel Wendlinger, Christiane Philippini Ferreira, Lívia Câmara de Carvalho Galvão, Alessandro Dourado Loguercio
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引用次数: 0
摘要
本体外研究旨在定量过氧化氢(HP)的渗透,漂白效果(BE)和不同漂白技术对幼虫的毒性。60颗上颌前磨牙分为6组(n = 10): Pola lumininate (PL)、VivaStyle Paint On PIus (VS)、PolaDay (PD)、White Class (WC)和Whiteness HP Automixx (AM)。未接触漂白剂的一组被评价为对照组(CG)。以前,漂白剂中HP的初始浓度是通过滴定法确定的,pH值是用数字pH计测量的。采用紫外可见分光光度法测定牙髓腔内HP的浓度(µg/mL)。用数字分光光度计测定BE (ΔE* ab和ΔE00)。采用幼虫模型进行毒性评价。HP穿透和颜色变化的数据采用单因素方差分析和Tukey检验,以及Kaplan-Meier死亡曲线对毒性进行评估(α = 0.05)。VS、PD和AM的初始浓度低于厂家报告的浓度。VS具有更强的酸性ph值。与VS相比,PL和WC的牙髓腔内HP含量显著降低(p = 0.001)。对于BE, ΔE* ab (p = 0.38)和ΔE00 (p = 0.42)无显著差异。6% HP与GC比较无毒性(p < 0.05)。所有6%的HP产品均显示漂白效果,对牙髓腔渗透低,对黄粉虫幼虫无毒。
The Effect of Different Bleaching Techniques Using 6% Hydrogen Peroxide: Penetration Inside the Pulp Cavity, Bleaching Efficacy and Toxicity.
This in vitro study aimed to quantify the penetration of hydrogen peroxide (HP), bleaching efficacy (BE) and toxicity in larvae in different bleaching techniques using 6% HP. Sixty maxillary premolars were divided in six groups (n = 10): Pola Luminate (PL), VivaStyle Paint On PIus (VS), PolaDay (PD), White Class (WC) and Whiteness HP Automixx (AM). A group not exposed to bleaching agents was evaluated as a control group (CG). Previously, the initial HP concentration in the bleaching agents was determined via titration and pH was measured with a digital pH meter. The concentration (µg/mL) of HP inside the pulp cavity was assessed using UV-Vis spectrophotometry. The BE (ΔE* ab and ΔE00) was evaluated with a digital spectrophotometer. The toxicity was evaluated in larvae model. Data from HP penetration and color change were evaluated for a one-way ANOVA and the Tukey's test, as well as Kaplan-Meier death curve for toxicity (α = 0.05). VS, PD and AM had an initial concentration lower than that the manufacturers reported. VS had a more acidic pH. PL and WC showed a significantly lower HP amount inside the pulp cavity when compared to VS (p = 0.001). Regarding BE, no significant difference was observed for ΔE* ab (p = 0.38), and ΔE00 (p = 0.42). No toxicity was observed when all 6% HP was compared to GC (p > 0.05). All 6% HP products tested showed bleaching efficacy, low penetration into the pulp chamber and were non-toxic to Tenebrio molitor larvae.