In vitro detection of antimicrobial impact, organic acids production and phytochemical screening of Musa acuminata wastes after treatment with fungi.

The current study was performed to evaluate the bioactivity of banana wastes against some bacterial and fungal isolates, organic acids production, antioxidant and to screen the phytochemicals were present in Musa acuminate (M. acuminate) wastes. The waste was processed by some microbial species. The organic acids were evaluated in the yield using HPLC. Antioxidant impact of the yield was tested by DPPH assay. Qualitative analysis for phytochemicals were done after each treatment. M. acuminate (Banana) extracts showed moderate effect against the growth of tested microorganisms while this effect became much better after treated with fungal species (Fusarium moniliform; Rhizopus stolonifer; Aspergillus parasiticus; Alternaria alternate; Penicillium expansum and Aspergillus flavus). The MIC ranging from 6.25 to 100 mg/ml, MBC and MFC ranging from 3.125 to 250 mg/ml. Citric acid was the most produced organic acid obtained from banana wastes and highly increased after treatment with fungal isolates used. The antioxidant potential of M. acuminata leaves and pseudostem wastes enhanced after treatment with fungal extracts relative to butylated hydroxytoluene (BHT). Phytochemical screening confirmed the presence of active compounds like glycosides, tannins, saponnins, phenols, steroids and flavonoids in the M. acuminate extracts. The treated banana wastes using fungi could be used as a source of in citric acid production, antioxidant source and other chemical production to be applied in many applications.