Molecular mechanism of response to low-temperature during the natural overwintering period of Rosa persica.

Key message: The JA and ICE-CBF-COR signaling pathways play important roles in the low-temperature response of Rosa persica, with RpMYC2 interacting with multiple transcription factors and positively regulating tolerance to low-temperature stress. Rosa persica is highly resilient to cold and drought, making it a valuable resource for breeding in the Rosa. However, the response mechanism of R. persica during the overwintering period remains unclear. This study examined root and stem tissues of R. persica over an eight-month natural open field overwintering period, measuring physiological indices of cold tolerance and investigating changes in cold tolerance across different overwintering stages. The values of physiological indicators of cold hardiness of R. persica roots and stems increased and then decreased. Osmoregulatory substances were the primary contributors to cold hardiness of R. persica roots, while antioxidant enzyme systems played a dominant role in cold hardiness of stems. Differential gene enrichment analyses revealed that oxidative reactions, the synthesis of various secondary metabolites, and hormone signaling pathways are crucial in establishing cold tolerance of R. persica at different overwintering stages. Weighted gene co-expression network and time-ordered gene co-expression network analyses identified the gene RpMYC2 as potentially key to cold tolerance in R. persica. Yeast two-hybrid discovery revealed that RpMYC2 interacts with multiple transcription factors to regulate cold stress resistance in R. persica. Based on the transcriptome, key genes involved in response to low temperature were identified in this study, providing the physiological and molecular insights for cold tolerance breeding of Rosa.