IF 3.1 4区 医学 Q3 IMMUNOLOGY
Xin Wang, Haojie Xu, Yuyan Chao, Chao Sun, Tingting Wang, Xiaoyun Fan, Lin Tang, Shengqian Xu, Changhao Xie
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引用次数: 0

摘要

目的 尽管人们越来越关注强直性脊柱炎(AS)患者的肠道微生物群和血液代谢组,但对其作用仍然知之甚少。在此,我们研究了微生物和代谢改变是如何导致强直性脊柱炎的。 方法 采用 16S 核糖体 RNA(rRNA)基因测序法,将 40 名强直性脊柱炎患者的粪便微生物组数据与 40 名健康对照者(HCs)的粪便微生物组数据进行比较。分析血浆代谢谱并将其与微生物群数据进行整合,以确定强直性脊柱炎特有的生物学特征。 结果 强直性脊柱炎患者表现出特定菌属的明显富集,包括Megamonas、Elusimicrobium、Dysgonomonas、Ruminococcus_gauvreauii_group和未分类的前伏牛花科(Prevotellaceae)。代谢物表达差异最大的途径包括胆汁分泌;新霉素、卡那霉素和庆大霉素的生物合成;花生四烯酸代谢。Megamonas和Elusimicrobium与吡贝地尔、L-胱硫醚和鳄梨甙二醛等代谢物之间的正相关表明强直性脊柱炎患者体内微生物丰富。 结论 强直性脊柱炎患者体内存在紊乱的肠道微生物群和改变的代谢物。微生物组和代谢组数据的整合揭示了强直性脊柱炎患者体内的重大紊乱,从而增进了我们对其发病机制的了解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Integration of Metabolomics and 16S Ribosomal RNA Sequencing to Elucidate the Pathogenesis of Ankylosing Spondylitis

Integration of Metabolomics and 16S Ribosomal RNA Sequencing to Elucidate the Pathogenesis of Ankylosing Spondylitis

Objective

Despite growing interest in the gut microbiota and blood metabolome in patients with ankylosing spondylitis (AS), its role remains poorly understood. Here, we investigate how microbial and metabolic alterations contribute to AS.

Methods

Fecal microbiome data from 40 AS patients were compared with those from 40 healthy controls (HCs) using 16S ribosomal RNA (rRNA) gene sequencing. The plasma metabolic profiles were analyzed and integrated with the microbiota data to identify biological characteristics specific to AS.

Results

AS patients showed significant enrichment of specific genera, including Megamonas, Elusimicrobium, Dysgonomonas, Ruminococcus_gauvreauii_group, and unclassified_Prevotellaceae. Pathways with the most differentially expressed metabolites included bile secretion; neomycin, kanamycin, and gentamicin biosynthesis; and arachidonic acid metabolism. Positive correlations between Megamonas and Elusimicrobium and metabolites such as piribedil, l-cystathionine, and crocetin dialdehyde suggested microbial enrichment in AS patients.

Conclusions

A disrupted gut microbiota and altered metabolites are present in AS patients. Integrating microbiome and metabolomic data reveals significant disruptions in AS patients, improving our understanding of its pathogenesis.

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来源期刊
Immunity, Inflammation and Disease
Immunity, Inflammation and Disease Medicine-Immunology and Allergy
CiteScore
3.60
自引率
0.00%
发文量
146
审稿时长
8 weeks
期刊介绍: Immunity, Inflammation and Disease is a peer-reviewed, open access, interdisciplinary journal providing rapid publication of research across the broad field of immunology. Immunity, Inflammation and Disease gives rapid consideration to papers in all areas of clinical and basic research. The journal is indexed in Medline and the Science Citation Index Expanded (part of Web of Science), among others. It welcomes original work that enhances the understanding of immunology in areas including: • cellular and molecular immunology • clinical immunology • allergy • immunochemistry • immunogenetics • immune signalling • immune development • imaging • mathematical modelling • autoimmunity • transplantation immunology • cancer immunology
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