177lu标记肺癌抗egfr Fab抗体的合成及评价

IF 1.5 3区化学 Q3 CHEMISTRY, ANALYTICAL

Journal of Radioanalytical and Nuclear Chemistry Pub Date : 2025-03-04 DOI:10.1007/s10967-025-10030-4

Yuan-Yuan Li, Jia-Yan Yu, Tao Wang, Jing Wang, Peng Zhao, Xia Yang, Hong-Yuan Wei, Yue Chen

{"title":"177lu标记肺癌抗egfr Fab抗体的合成及评价","authors":"Yuan-Yuan Li, Jia-Yan Yu, Tao Wang, Jing Wang, Peng Zhao, Xia Yang, Hong-Yuan Wei, Yue Chen","doi":"10.1007/s10967-025-10030-4","DOIUrl":null,"url":null,"abstract":"<div><p>Fab fragments obtained via enzymatic digestion of full-length antibodies or by gene recombination technology have smaller molecular weight and greater penetration ability. Those advantages make it uniquely suited to deliver radionuclides. In this study, we prepared a Fab fragment from the anti-EGFR antibody Cetuximab and labeled it with <sup>177</sup>Lu. The binding activity in vitro and biodistribution in vivo of [<sup>177</sup>Lu]Lu-DOTA-Fab-Cetuximab were investigated. The [<sup>177</sup>Lu]Lu-labeled Fab fragment showed improved tumor penetration and faster blood clearance compared to the full-length antibody.</p></div>","PeriodicalId":661,"journal":{"name":"Journal of Radioanalytical and Nuclear Chemistry","volume":"334 3","pages":"2139 - 2149"},"PeriodicalIF":1.5000,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Synthesis and evaluation of 177Lu-labeled anti-EGFR Fab antibody for lung cancer\",\"authors\":\"Yuan-Yuan Li, Jia-Yan Yu, Tao Wang, Jing Wang, Peng Zhao, Xia Yang, Hong-Yuan Wei, Yue Chen\",\"doi\":\"10.1007/s10967-025-10030-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Fab fragments obtained via enzymatic digestion of full-length antibodies or by gene recombination technology have smaller molecular weight and greater penetration ability. Those advantages make it uniquely suited to deliver radionuclides. In this study, we prepared a Fab fragment from the anti-EGFR antibody Cetuximab and labeled it with <sup>177</sup>Lu. The binding activity in vitro and biodistribution in vivo of [<sup>177</sup>Lu]Lu-DOTA-Fab-Cetuximab were investigated. The [<sup>177</sup>Lu]Lu-labeled Fab fragment showed improved tumor penetration and faster blood clearance compared to the full-length antibody.</p></div>\",\"PeriodicalId\":661,\"journal\":{\"name\":\"Journal of Radioanalytical and Nuclear Chemistry\",\"volume\":\"334 3\",\"pages\":\"2139 - 2149\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2025-03-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Radioanalytical and Nuclear Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10967-025-10030-4\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Radioanalytical and Nuclear Chemistry","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s10967-025-10030-4","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

摘要

通过酶切全长抗体或基因重组技术获得的Fab片段分子量更小，穿透能力更强。这些优点使它特别适合运送放射性核素。在本研究中，我们从抗egfr抗体西妥昔单抗中制备了Fab片段，并用177Lu标记。研究了[177Lu]Lu-DOTA-Fab-Cetuximab的体外结合活性和体内生物分布。与全长抗体相比，[177Lu] lu标记的Fab片段表现出更好的肿瘤穿透性和更快的血液清除。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Synthesis and evaluation of 177Lu-labeled anti-EGFR Fab antibody for lung cancer

查看原文本刊更多论文

Synthesis and evaluation of 177Lu-labeled anti-EGFR Fab antibody for lung cancer

Fab fragments obtained via enzymatic digestion of full-length antibodies or by gene recombination technology have smaller molecular weight and greater penetration ability. Those advantages make it uniquely suited to deliver radionuclides. In this study, we prepared a Fab fragment from the anti-EGFR antibody Cetuximab and labeled it with ¹⁷⁷Lu. The binding activity in vitro and biodistribution in vivo of [¹⁷⁷Lu]Lu-DOTA-Fab-Cetuximab were investigated. The [¹⁷⁷Lu]Lu-labeled Fab fragment showed improved tumor penetration and faster blood clearance compared to the full-length antibody.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of Radioanalytical and Nuclear Chemistry 化学-分析化学

CiteScore

2.80

自引率

18.80%

发文量

504

审稿时长

2.2 months

期刊介绍： An international periodical publishing original papers, letters, review papers and short communications on nuclear chemistry. The subjects covered include: Nuclear chemistry, Radiochemistry, Radiation chemistry, Radiobiological chemistry, Environmental radiochemistry, Production and control of radioisotopes and labelled compounds, Nuclear power plant chemistry, Nuclear fuel chemistry, Radioanalytical chemistry, Radiation detection and measurement, Nuclear instrumentation and automation, etc.