甘草酸对脂磷胆酸和脂多糖诱导的牛乳腺炎的抗炎作用。

T Kurumisawa, K Kazama, S Gondaira, H Higuchi, A Eguchi, K Onda, S-G Roh, K Kawai
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引用次数: 0

摘要

牛乳腺炎主要用抗菌剂治疗。抗炎药也用于缓解临床症状或减少抗菌药物的使用。甘草酸是一种用于治疗牛乳腺炎的抗炎剂,但其作用尚不完全清楚。因此,我们研究了甘草酸在体内和体外的抗炎作用。我们首先使用永生化牛乳腺上皮细胞系MAC-T细胞测试了甘草酸是否具有抗炎作用。甘草酸在脂磷胆酸(LTA)刺激下降低白细胞介素(IL)-1β mRNA的表达,并呈浓度依赖性。然后,我们研究了甘草酸对牛乳腺上皮细胞(bMECs)的影响,这些细胞似乎保留了更多实际乳腺上皮细胞的特征。LTA或脂多糖刺激可显著增加bmec细胞因子mRNA的表达。甘草酸有轻微的抑制作用,但无显著性差异。研究了甘草酸对乳牛lta性乳腺炎的影响。每个季度分为试验区和控制区(试验区:n=8,对照区:n=7)。在试验开始时(0 h),所有四分之一的小鼠都接受LTA刺激。在试验组中,甘草酸通过乳内输注给予。试验区小鼠体细胞计数、IL-1β、肿瘤坏死因子-α相关基因表达量均显著低于对照组。体外和体内研究均表明,甘草酸在lta诱导的炎症反应中可降低促炎细胞因子基因的表达,部分揭示了甘草酸对乳腺炎抗炎作用的机制。需要对乳腺炎合并细菌感染的现场病例进行进一步的调查,以证明甘草酸苷对牛乳腺炎的抗炎作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Anti-inflammatory effects of glycyrrhizin on lipoteichoic acid and lipopolysaccharide-induced bovine mastitis.

Bovine mastitis is primarily treated with antimicrobial agents. Anti-inflammatory agents are also used to alleviate clinical symptoms or reduce antimicrobial use. Glycyrrhizin is an anti-inflammatory agent used in the treatment of bovine mastitis, but its effects are not fully understood. We therefore examined the anti-inflammatory effects of glycyrrhizin both in vivo and in vitro. We first tested whether glycyrrhizin exerts anti-inflammatory effects using MAC-T cells, an immortalized bovine mammary epithelial cell line. Glycyrrhizin decreased the expression of interleukin (IL)-1β mRNA in a concentration-dependent manner in MAC-T cells stimulated with lipoteichoic acid (LTA). We then investigated the effects of glycyrrhizin in bovine mammary epithelial cells (bMECs), which seem to retain more of the characteristics of actual mammary epithelial cells. Stimulation with LTA or lipopolysaccharide significantly increased cytokine mRNA expression in bMECs. Glycyrrhizin exhibited a slight inhibitory effect, but no significant difference was observed. The effect of glycyrrhizin on LTA-induced mastitis was examined in lactating cows. Quarters were divided into test and control areas (test quarter: n=8, control quarter: n=7). All quarters were stimulated with LTA at the start of the trial (0 h). In the test quarter group, glycyrrhizin was administered via intramammary infusion. The somatic cell count and relative gene expression of IL-1β and tumor necrosis factor-α were significantly lower in test quarters than control quarters. Both the in vitro and in vivo studies showed that glycyrrhizin reduces the expression of proinflammatory cytokine genes in response to LTA-induced inflammation and partially revealed the mechanism of the anti-inflammatory effect of glycyrrhizin on mastitis. Further investigations involving field cases of mastitis with bacterial infections are needed to demonstrate the anti-inflammatory effect of glycyrrhizin on bovine mastitis.

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