Saccharum复合体中cad样家族基因在生物和非生物胁迫下的功能分化

IF 5.3 2区 生物学 Q1 PLANT SCIENCES
Zhu-Qing Wang, Sheng-Ren Sun, Hao-Yu Wang, Zheng-Wang Bi, Hai-Long Chang, Shuo-Hang Zhang, Jun-Lv Chen, Yuan-Xia Qin, Jian-Tao Wu, Wei Zhang, Huan-Ying Xu, San-Ji Gao, Qin-Nan Wang
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引用次数: 0

摘要

在Saccharum complex中共鉴定出54个膜攻击复合体/穿孔素(MACPF)超家族基因,并且在植物抗胁迫中存在ssacad样基因之间的功能差异。膜攻击复合物/穿孔蛋白(MACPF)超家族属于真核生物先天免疫和适应性免疫中涉及的成孔蛋白。含有MACPF结构域的组成性活化细胞死亡(CAD)蛋白参与逆境条件下的植物防御反应。然而,甘蔗中cad样基因的特性和功能仍然知之甚少。本研究从甘蔗(Saccharum spontanum) 2个克隆(Np-X和AP85-441)和Erianthus rufipilus 1个克隆(Yunnan2009-3) 3个基因组中鉴定出54个cad样基因。所有cad样基因可分为5个系统发育类群(I-V)。在启动子区域发现了多种与胁迫反应相关的顺式作用元件,如植物激素反应元件。转录组和RT-qPCR分析表明这些基因具有不同的表达谱。在甘蔗品种中,SsaCAD1-like1基因在冷处理和白色黄单胞菌(Xanthomonas albilineans, Xa)侵染导致叶片烫伤后表达上调。同时,该基因在干旱和ABA处理下均表现为下调,但在SA处理的不同时间点上均呈现先上调后下调的趋势。SsaCAD4-like1基因在5种非生物应激源下下调。SsaCAD2-like1/2等位基因在除盐度处理外的所有非生物胁迫下的表达水平均显著降低。3个等位基因(SsaCAD3-like1/2/4)在非生物胁迫下的表达模式相似。在转基因拟南芥系中,SsaCAD1-like1表现为负作用,而SsaCAD3-like2/4表现为正作用。本研究结果为甘蔗抗病品种的培育提供了新的基因资源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Functional divergence of CAD-like family genes in Saccharum complex under biotic and abiotic stress.

Key message: A total of 54 genes of membrane attack complex/perforin (MACPF) superfamily were identified in Saccharum complex and function divergence among SsaCAD-like genes were present in plant against stressors. The membrane attack complex/perforin (MACPF) superfamily belongs to pore-forming proteins involving in innate and adaptive immunity in eukaryotes. The constitutively activated cell death (CAD) proteins contained the MACPF domain participate in plant defense responses under adverse conditions. However, the characteristics and functions of CAD-like genes in sugarcane are still poorly understood. In this study, 54 CAD-like genes were identified in three genomes from Saccharum complex including two clones (Np-X and AP85-441) of Saccharum spontaneum and a clone (Yunnan2009-3) of Erianthus rufipilus. All CAD-like genes were categorized into five phylogenetic groups (I-V). Various cis-acting elements related to stress responses, such as phytohormone response elements, were found in promoter regions. Transcriptome and RT-qPCR analysis demonstrated these genes possessing diverse expression profiles. The SsaCAD1-like1 gene was upregulated in sugarcane cultivars after cold treatment and infection by Xanthomonas albilineans (Xa) causing leaf scald. Meanwhile, this gene was downregulated under drought and ABA treatments but was upregulated and then downregulated across time-points of SA treatment. The SsaCAD4-like1 gene was downregulated under five abiotic stressors. Expression levels of two alleles (SsaCAD2-like1/2) were significantly decreased under all abiotic stressors except for salinity treatment. Similar expression patterns of three alleles (SsaCAD3-like1/2/4) were found under abiotic stress. The SsaCAD1-like1 exhibited a negative role but SsaCAD3-like2/4 acted as positive roles in transgenic Arabidopsis lines against bacterial pathogen infection. Our results provide novel gene resources for developing disease-resistant cultivars in sugarcane.

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来源期刊
Plant Cell Reports
Plant Cell Reports 生物-植物科学
CiteScore
10.80
自引率
1.60%
发文量
135
审稿时长
3.2 months
期刊介绍: Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as: - genomics and genetics - metabolism - cell biology - abiotic and biotic stress - phytopathology - gene transfer and expression - molecular pharming - systems biology - nanobiotechnology - genome editing - phenomics and synthetic biology The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.
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