Matthew K Schnizlein, Abhishek A Dubey, Aretha Fiebig, Sean Crosson
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Genetic- and culture-based tools for studying Bacteroides fragilis.
The relatively limited availability of genetic tools has hampered mechanistic studies of Bacteroides fragilis, an opportunistic anaerobe that constitutes 1%-5% of the gut microbiota in healthy humans. Here we describe novel vectors for B. fragilis gene deletion and expression as well as a semi-defined media for cultivation of B. fragilis str. P207.