烟酰胺单核苷酸、德乌尔素和l-半胱氨酸的混合物在体外降低衰老相关标志物,并对2,4-二硝基氯苯诱导的特应性皮炎有效:网络药理学的应用。

IF 1.7 3区 农林科学 Q4 CHEMISTRY, MEDICINAL
Hyo Ri Shin, La Yoon Choi, Seong Hwan Bae, Tae Gyun Kim, Hyung Taek Cho, Tae Jin Cho, Nam Su Oh, Youn Young Shim, Martin J T Reaney, Jeong Hoon Pan, Jeong-Oh Shin, Eui-Cheol Shin, Seong-Gyu Ko, Jinbong Park, Yohan Han, Kyung-Chul Choi, Young Jun Kim, Wan Heo, Kangwook Lee, Jae Kyeom Kim
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引用次数: 0

摘要

环境因素造成的氧化应激加速了皮肤老化,导致胶原蛋白和弹性蛋白分解,产生皱纹和炎症等明显迹象。天然抗氧化剂,如烟酰胺单核苷酸(NMN)、乙酰胆碱和l-半胱氨酸,已显示出对抗氧化损伤和炎症的潜力。本研究通过体外、体内和计算机方法的结合,探讨了这些化合物的配方混合物对皮肤老化和特应性皮炎(AD)的影响。使用人角质形成细胞,我们通过细胞活力测定评估细胞毒性,确认NMN和l-半胱氨酸在100µM以下无毒,而前尿素在10µM以上表现出毒性。细胞内氧化应激测定结果表明,由相同浓度的前尿素和l-半胱氨酸组成的混合物A和B的NMN含量不同(混合物A低,混合物B中等),可显著降低2,2'-偶氮(2-脒基丙烷)二盐酸诱导的氧化应激水平,而含有最高NMN浓度的混合物C则无效。混合物B进一步减少氧化应激下与衰老相关的异染色质病灶的形成,而混合物C则导致细胞结构破坏。在二硝基氯苯诱导的BALB/c小鼠AD模型中,混合物- l和混合物- h处理均降低了表皮厚度、抓伤行为和经皮失水,其中混合物- l还降低了皮肤厚度和肥大细胞浸润。基因表达分析证实混合物B降低了TNF-α和IL-6等促炎细胞因子,而网络药理学预测了关键的抗氧化途径,通过恢复NOS2基因表达进行了验证。总的来说,研究结果强调了这些化合物混合物在减轻氧化应激和炎症方面的潜力,为皮肤老化和皮炎治疗提供了一种有希望的方法,尽管需要进一步验证以优化功效和安全性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A Mixture of Nicotinamide Mononucleotide, Decursin, and l-Cysteine Lowered Senescence-Associated Markers In Vitro and Was Effective Against 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis In Vivo: An Application of Network Pharmacology.

Skin aging, accelerated by oxidative stress from environmental factors, results in the breakdown of collagen and elastin, causing visible signs like wrinkles and inflammation. Natural antioxidants such as nicotinamide mononucleotide (NMN), decursin, and l-cysteine have shown potential in combating oxidative damage and inflammation. This study explored the effects of a formulated mixture of these compounds on skin aging and atopic dermatitis (AD) through a combination of in vitro, in vivo, and in silico methods. Using human keratinocyte cells, we assessed cytotoxicity via a cell viability assay, confirming NMN and l-cysteine were nontoxic up to 100 µM, while decursin exhibited toxicity above 10 µM. The intracellular oxidative stress measurement demonstrated that mixtures A and B, composed of the same concentrations of decursin and l-cysteine but differing in NMN levels (low in mixture A and moderate in mixture B), significantly reduced oxidative stress levels induced by 2,2'-azobis(2-amidinopropane) dihydrochloride, whereas mixture C, which contained the highest NMN concentration, was ineffective. Mixture B further reduced senescence-associated heterochromatin foci formation under oxidative stress, while mixture C caused cell structure disruptions. In the dinitrochlorobenzene-induced AD model in BALB/c mice, both, mixture-L and mixture-H treatments reduced epidermal thickness, scratching behavior, and transepidermal water loss, with mixture-L also lowering dermal thickness and mast cell infiltration. Gene expression analysis confirmed that mixture B decreased proinflammatory cytokines like TNF-α and IL-6, while network pharmacology predicted key antioxidant pathways, validated through restored NOS2 gene expression. Overall, the findings highlight the potential of these compound mixtures to mitigate oxidative stress and inflammation, offering a promising approach for skin aging and dermatitis management, though further validation is needed to optimize efficacy and safety.

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来源期刊
Journal of medicinal food
Journal of medicinal food 医学-食品科技
CiteScore
4.50
自引率
0.00%
发文量
154
审稿时长
4.5 months
期刊介绍: Journal of Medicinal Food is the only peer-reviewed journal focusing exclusively on the medicinal value and biomedical effects of food materials. International in scope, the Journal advances the knowledge of the development of new food products and dietary supplements targeted at promoting health and the prevention and treatment of disease.
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