Establishment and Application of Time-Resolved Fluorescence Strip Method for Biotin Detection in Milk Powder

Biotin can be added to milk powder to achieve recommended daily intake levels. In order to determine whether the actual amount of biotin added in food is consistent with the labeled content, a time-resolved fluorescence immunochromatographic assay (TRFICA) was established based on monoclonal antibodies for biotin detection in milk powder with a detection limit of 33.70 μg/kg, a relative recovery rate of 98.43%–100.38%, and a coefficient of variation of 3.36%–10.77%. After comparing the detection results of the two methods, we found that the proposed TRFICA results were consistent with the microbiological quantification method for vitamins, thus confirming the accuracy of the method. The detection time of the TRFICA was about 15 min, demonstrating rapid on-site detection and quantification of biotin concentrations in milk powder.