Metal-based nanoenzymes with excellent biocompatibility and stable chemical properties are an effective antimicrobial agent against bacterial resistance due to their radical-mediated catalysis. In this work, due to the pH of most bacterial infection sites being close to neutral, targeting the problem of Fe3O4@Ag difficulty in maintaining the catalytic activity of nanoenzymes in neutral environments, we prepare a novel multifunctional Fe3O4@Ag@ indocyanine green/adenosine triphosphate peroxidase nanoenzymes for synergistic antibacterial activity. ICG (Indocyanine Green) and ATP (Adenosine triphosphate) are adsorbed on the surface of Fe3O4@Ag through electrostatic adsorption to form its structure. The cell viability remained above 90%, indicating its good biocompatibility. By complexing ATP with nanoenzymes to participate in single electron transfer and binding with Fe (II), ATP promotes the sudden release of hydroxyl radical (·OH) from the system, successfully transferring Fe3O4@Ag the peroxidase activity of nanoenzymes extends to neutral pH. By utilizing ICG as a photosensitizer and a sonosensitizer, under the combined treatment of near-infrared light and ultrasound, the photodynamic therapy (PDT)/photothermal therapy (PTT)/sonodynamic therapy (SDT) functions can be achieved, achieving multifunctional synergistic antibacterial effects. In a neutral environment, its bactericidal efficiency against Gram negative (Escherichia coli) and Gram positive (Staphylococcus aureus) is 99.9% and 99.7%, respectively, providing a new multi-mode synergistic antibacterial strategy for bacterial infections.
Metal based nanoenzymes with excellent biocompatibility and stable chemical properties are an effective antimicrobial agent against bacterial resistance due to their radical mediated catalysis. The prepared multifunctional Fe3O4@Ag@ICG/ATP exhibit peroxidase nanoenzymes perform ance for synergistic antibacterial activity. ICG and ATP are adsorbed on the surface of Fe3O4@Ag through electrostatic adsorption to form its structure