通过对生理组、代谢组和转录组的综合分析，深入了解蟹爪兰花青素的生物合成和降解过程

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-03-22 DOI:10.1016/j.plaphy.2025.109821

Tiantian Sun , Mengzhu Wang , Hongfang Ren , Qingqing Xiong , Jianfeng Xu , Xiaoqian Yang , Yongxia Chen , Wangxiang Zhang

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引用次数: 0

摘要

蟹爪兰因其观赏性和园林应用而备受推崇，其叶片颜色变化是美学吸引力的重要指标。尽管如此，有关蟹爪兰叶色变化，尤其是紫红色色调褪去的研究仍然有限。本研究通过生理、转录和新陈代谢测定，研究了驱动蟹爪兰叶色变化的生理和分子机制。研究分析了 86 个蟹爪兰品种的叶色，并选择了三个处于不同颜色发展路径（从幼叶到成熟期的颜色变化）的代表性品种，对黄酮类生物合成途径中的基因表达和代谢物积累进行了详细研究。我们的研究结果表明，与成熟叶片相比，嫩叶的变化更大，与 "绿变青"（G-G）和 "紫变绿"（P-G）相比，"紫变紫"（P-P）颜色路径的稳定性更高。综合分析强调了花青素，尤其是绿色蟹爪兰叶片中的花青素（pelargonidin）和芍药苷（peonidin 3-glucoside）以及紫红色蟹爪兰叶片中的花青素（cyanidin），是调节叶片颜色的核心成分。转录组分析表明，紫红色的褪色归因于花青素总积累的减少和花青素的降解。这一过程受花青素合成酶（ANS）基因下调和花青素降解基因过氧化物酶（PRX）上调的调控。此外，还发现了两个可能参与调控花青素生物合成的转录因子和两个调控茄红素生物合成的转录因子。这项研究确定了影响紫红色叶片花青素积累的候选基因，为今后研究叶片着色机制和蟹爪兰育种工作奠定了基础。

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Comprehensive analysis of the physiological, metabolome, and transcriptome provided insights into anthocyanin biosynthesis and degradation of Malus crabapple

Malus crabapple is highly regarded for its ornamental and garden applications, with leaf color changes serving as an essential indicator of aesthetic appeal. Despite this significance, studies focusing on crabapple leaf color transformations, particularly the fading of purplish-red hues, remain limited. This research investigates the physiological and molecular mechanisms driving leaf color changes in crabapple through physiological, transcriptional, and metabolic assays. Leaf color was analyzed across 86 crabapple varieties, with three representative varieties in different color development paths (the color change from young to mature stage) selected for detailed examination of gene expression and metabolite accumulation within the flavonoid biosynthetic pathway. Our findings revealed greater variation in young leaves compared to mature ones, along with higher stability in the ‘Purple to Purple’ (P-P) color path compared to the ‘Green to Green’ (G-G) and ‘Purple to Green’ (P-G) paths. The comprehensive analysis highlighted anthocyanins, particularly pelargonidin and peonidin 3-glucoside in green crabapple leaves and cyanidin in purplish-red crabapple leaves, as central to leaf color regulation. Transcriptomic analysis revealed that the fading of purplish-red is attributable to decreased accumulation of total anthocyanin and degradation of cyanidin. This process is governed by the down-regulation of anthocyanidin synthase (ANS) gene and the up-regulation of the anthocyanin degradation gene, peroxidase (PRX). Additionally, two transcription factors potentially involved in the regulation of cyanidin biosynthesis and two transcription factors regulating pelargonidin biosynthesis were identified. This study identifies candidate genes influencing anthocyanin accumulation in purplish-red leaves, providing a foundation for future investigations into leaf coloration mechanisms and crabapple breeding efforts.

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.