利用DC-EPG系统分析毛地黄蚜虫对大豆基因型的探测行为

IF 2.5 2区农林科学 Q1 AGRONOMY

Crop Protection Pub Date : 2025-03-21 DOI:10.1016/j.cropro.2025.107210

Samuel A. Fasusi , Bo Yoon Seo , Hong Min Koh , Ji-Min Kim , Sungtaeg Kang

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引用次数: 0

摘要

毛地黄蚜（Aulacorthum solani）是一种威胁粮食安全的大豆害虫。然而，在韩国和日本，对大豆植物对FAs的抗性研究较少。了解FAs如何渗透到大豆植物组织中，并确定其抗性的位置，对于有效地管理害虫至关重要。本研究利用电渗透图（EPG）技术评价了FAs对3种易感和5种（中度和高度）抗性大豆基因型的探测行为。我们利用EPG可视化了蚜虫柱头在大豆组织中的运动和探测行为。我们仔细研究了14小时喂养过程中产生的8种不同波形。易感基因型非探测波持续时间和频率较短，E2波持续时间和频率较高。相比之下，中度和高度耐药基因型产生较少的E2波形，但产生更多的np波形。抗性基因型E2波形值的频率和持续时间较低，表明抗性因子位于韧皮部。因此，韧皮部防御机制抑制FA活性。这项开创性的研究揭示了FAs在不同大豆基因型中的探测行为。了解不同基因型中FA探测行为的变化有助于阐明FA抗性的机制，并可以指导未来植物与昆虫相互作用的研究，以帮助选择抗性基因型。

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Probing behavior analysis of foxglove aphids on soybean genotypes using the DC-EPG system

The foxglove aphid (FA), Aulacorthum solani, is a soybean pest that threatens food security. However, few studies on soybean plant resistance to FAs with resistance to Aulacorthum solani (Raso) genes have been conducted in Korea and Japan. Understanding how FAs infiltrate soybean plant tissues and identifying the location of resistance are crucial for the effective management of insect pests. This study focused on evaluating the probing behavior of FAs that fed on three susceptible and five (moderately and highly) resistant soybean genotypes using the electrical penetration graph (EPG) technique. We used EPG to visualize the movement of the aphid stylet and probing behavior in soybean tissues. We carefully studied the eight distinct waveforms generated during 14 h of feeding. The susceptible genotypes exhibited shorter duration and frequency of non-probing (np) waveforms but higher duration and frequency of E2 waveforms. In contrast, moderately and highly resistant genotypes produced fewer E2 waveforms but more np waveforms. The lower frequency and duration of the E2 waveform values from the resistant genotypes indicated that the resistance factor was located in the phloem. Therefore, phloem defense mechanisms inhibit FA activity. This pioneering study characterized the probing behavior of FAs in various soybean genotypes. Understanding the variations in FA probing behavior across different genotypes is useful for elucidating the mechanism of FA resistance and can guide future research on plant-insect interactions to assist in selecting resistant genotypes.

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来源期刊

Crop Protection 农林科学-农艺学

CiteScore

6.10

自引率

3.60%

发文量

200

审稿时长

29 days

期刊介绍： The Editors of Crop Protection especially welcome papers describing an interdisciplinary approach showing how different control strategies can be integrated into practical pest management programs, covering high and low input agricultural systems worldwide. Crop Protection particularly emphasizes the practical aspects of control in the field and for protected crops, and includes work which may lead in the near future to more effective control. The journal does not duplicate the many existing excellent biological science journals, which deal mainly with the more fundamental aspects of plant pathology, applied zoology and weed science. Crop Protection covers all practical aspects of pest, disease and weed control, including the following topics: -Abiotic damage- Agronomic control methods- Assessment of pest and disease damage- Molecular methods for the detection and assessment of pests and diseases- Biological control- Biorational pesticides- Control of animal pests of world crops- Control of diseases of crop plants caused by microorganisms- Control of weeds and integrated management- Economic considerations- Effects of plant growth regulators- Environmental benefits of reduced pesticide use- Environmental effects of pesticides- Epidemiology of pests and diseases in relation to control- GM Crops, and genetic engineering applications- Importance and control of postharvest crop losses- Integrated control- Interrelationships and compatibility among different control strategies- Invasive species as they relate to implications for crop protection- Pesticide application methods- Pest management- Phytobiomes for pest and disease control- Resistance management- Sampling and monitoring schemes for diseases, nematodes, pests and weeds.