IF 81.3 1区 生物学 Q1 CELL BIOLOGY
Katie W. Downes, Giulia Zanetti
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引用次数: 0

摘要

真核生物中有三分之一的蛋白质在内质网(ER)和高尔基体之间转运,以到达细胞内外的功能目的地。在输出过程中,分泌蛋白集中在ER的过渡区,即ER出口位点,在那里它们被包装成由高度保守的衣壳蛋白复合体II(COPII)形成的运输载体。尽管我们对早期分泌途径中的许多基本运输途径早已有所了解,但仍然缺乏一个完整的机理模型来解释 COPII 介导的ER出口的各个步骤是如何被调控以高效运输各种货物的。在这篇综述中,我们讨论了目前对 COPII 介导的囊泡转运机制的理解,并强调了尚未解决的知识空白。我们将重点关注包被的组装和拆卸如何决定载体的形态发生,COPII 如何选择性地招募大量货物和货物适配体,最后讨论哺乳动物中的 COPII 机制如何适应大型蛋白质的运输。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Mechanisms of COPII coat assembly and cargo recognition in the secretory pathway

Mechanisms of COPII coat assembly and cargo recognition in the secretory pathway

One third of all proteins in eukaryotes transit between the endoplasmic reticulum (ER) and the Golgi to reach their functional destination inside or outside of the cell. During export, secretory proteins concentrate at transitional zones of the ER known as ER exit sites, where they are packaged into transport carriers formed by the highly conserved coat protein complex II (COPII). Despite long-standing knowledge of many of the fundamental pathways that govern traffic in the early secretory pathway, we still lack a complete mechanistic model to explain how the various steps of COPII-mediated ER exit are regulated to efficiently transport diverse cargoes. In this Review, we discuss the current understanding of the mechanisms underlying COPII-mediated vesicular transport, highlighting outstanding knowledge gaps. We focus on how coat assembly and disassembly dictate carrier morphogenesis, how COPII selectively recruits a vast number of cargo and cargo adaptors, and finally discuss how COPII mechanisms in mammals might have adapted to enable transport of large proteins.

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来源期刊
Nature Reviews Molecular Cell Biology
Nature Reviews Molecular Cell Biology 生物-细胞生物学
CiteScore
173.60
自引率
0.50%
发文量
118
审稿时长
6-12 weeks
期刊介绍: Nature Reviews Molecular Cell Biology is a prestigious journal that aims to be the primary source of reviews and commentaries for the scientific communities it serves. The journal strives to publish articles that are authoritative, accessible, and enriched with easily understandable figures, tables, and other display items. The goal is to provide an unparalleled service to authors, referees, and readers, and the journal works diligently to maximize the usefulness and impact of each article. Nature Reviews Molecular Cell Biology publishes a variety of article types, including Reviews, Perspectives, Comments, and Research Highlights, all of which are relevant to molecular and cell biologists. The journal's broad scope ensures that the articles it publishes reach the widest possible audience.
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