海藻糖、葡糖醇、辅酶Q10和维生素E复合制剂在碱实验模型中的有效性和优越性的进一步评价。

IF 3 2区医学 Q1 OPHTHALMOLOGY

Experimental eye research Pub Date : 2025-03-22 DOI:10.1016/j.exer.2025.110357

Yucel Yigit , Gozde Sahin Vural , Yurdagul Girgin , Basak Isildar , Pakize Nur Akkaya , Gamze Tanriverdi , Muhammed Dara Tas , Ozlem Barut Selver

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引用次数: 0

摘要

目的：本研究的目的是在实验碱模型中对透明质酸（HA）组合制剂的疗效和比较优势进行更全面的评价。方法：对22只新西兰兔右眼进行实验性化学烧伤。然后将眼睛分为四组进行后续分析：海藻糖+HA (T组，n=6)；葡聚糖醇+HA (P组，n=6)；辅酶Q10+维生素E+HA (V组，n=6)；对照组（CG, n=4）。结果：第1、3、5天各组间上皮缺损面积大小差异无统计学意义。然而，观察到上皮缺损愈合的显着差异。组织学分析显示，P组在间质水肿、上皮破坏和淋巴浸润方面的愈合效果最佳。P组和v组α SMA （α光滑肌动蛋白）阳性区域更高。电镜检查显示，与对照组相比，治疗组前表面上皮形成和胶原排列增强。结论：本研究结果与文献中其他研究结果一致，海藻糖、葡聚糖醇、辅酶Q10均优于对照组。此外，组织学和免疫组织化学评估显示，P和V组比T组表现出更好的愈合迹象。值得注意的是，我们的研究首次比较分析了海藻糖、葡聚糖醇和辅酶Q10在上皮缺陷愈合方面的作用，并结合了电镜检查。

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Further evaluation of the effectiveness and superiority of hyaluronic acid combined preparations (trehalose, dexpanthenol and coenzyme Q10 & vitamin E) in experimental alkali model

Purpose

The objective of this study was to undertake a more comprehensive evaluation of the efficacy and comparative advantage of hyaluronic acid (HA) combined preparations in an experimental alkali model.

Method

In the present study, experimental chemical burns were induced in the right eyes of 22 New Zealand rabbits. The eyes were then divided into four groups for subsequent analysis: Trehalose + HA (Group T, n = 6); dexpanthenol + HA (Group P, n = 6); coenzyme Q10+vitamin E + HA (Group V, n = 6); and one control group (CG, n = 4).

Results

There was no statistically significant difference in the size of the epithelial defect area between the groups on days 1, 3, and 5. However, a marked difference in the healing of epithelial defects was observed. Histologic analysis revealed optimal healing in terms of stromal edema, epithelial disruption, and lymphatic infiltration in group P. Alpha SMA (alpha smooth actin)-positive areas was higher in groups P and V. Electron microscopic examination revealed enhanced anterior surface epithelial formation and collagen arrangement in the treatment groups compared to the control group.

Conclusion

The findings of the present study are consistent with those of other studies in the literature, which have shown the superiority of trehalose, dexpanthenol, and coenzyme Q10 to the control group. Furthermore, histologic and immunohistochemical evaluations revealed that the P and V groups exhibited superior healing signs in comparison to the T group. Notably, our study is the first to comparatively analyze artificial tears with trehalose, dexpanthenol, and coenzyme Q10 in terms of epithelial defect healing, incorporating electron microscopic examination.

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来源期刊

Experimental eye research 医学-眼科学

CiteScore

6.80

自引率

5.90%

发文量

323

审稿时长

66 days

期刊介绍： The primary goal of Experimental Eye Research is to publish original research papers on all aspects of experimental biology of the eye and ocular tissues that seek to define the mechanisms of normal function and/or disease. Studies of ocular tissues that encompass the disciplines of cell biology, developmental biology, genetics, molecular biology, physiology, biochemistry, biophysics, immunology or microbiology are most welcomed. Manuscripts that are purely clinical or in a surgical area of ophthalmology are not appropriate for submission to Experimental Eye Research and if received will be returned without review.