下利气胶囊通过NLRP3/Caspase-1通路调节前列腺上皮细胞凋亡治疗慢性前列腺炎的作用

IF 2.1 4区 医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
American Journal of Men's Health Pub Date : 2025-03-01 Epub Date: 2025-03-24 DOI:10.1177/15579883251322958
Zhiming Hong, Zilong Chen, Wenshuo Chen, Junfeng Qiu, Shengjie Wang, Yi Wei, Jun Guo
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引用次数: 0

摘要

慢性前列腺炎(CNP)是一种常见的男性炎症性疾病。下利气胶囊有缓解CNP患者临床症状的报道,可能与其抗炎作用有关;然而,其确切的作用机制尚不清楚。本研究将人正常前列腺上皮细胞(RWPE-2细胞)分为对照组、模型组、抑制剂组以及高、中、低含药血清组(分别为5%、10%、15%)。除对照组外,采用脂多糖(100 ng/mL)和三磷酸腺苷(5 mM)刺激形成细胞焦亡模型。随后,采用含药血清和nod样受体3 (NLRP3)抑制剂(MCC950)对模型细胞进行分组干预。在给药MCC950和含药血清后,抑制剂组和中高剂量组的细胞活力有所改善(分别提高20.5%、38.2%和73.2%)。透射电镜显示细胞焦亡特征减少。细胞上清中一氧化氮、白细胞介素-18 (IL-18)、肿瘤坏死因子-α水平显著降低(60.7%、21.6%、33.7%、41.8%;49.2%, 54.8%, 53.5%, 69.3%;31.3%, 44.4%, 38.1%, 51.2%)。免疫荧光显示NLRP3和半胱氨酸天冬氨酸蛋白酶1 (Caspase-1)蛋白荧光强度降低,Western Blot分析显示NLRP3、前Caspase-1和gasdermin D的表达显著下降(20.5%,45.9%,58.1%,74.8%;23.2%、36.7%、51.6%、51.9%;15.4%, 28.6%, 33.1%, 39.2%)。体外实验提示下利气胶囊可能通过抑制NLRP3/Caspase-1信号通路调节前列腺上皮细胞焦亡,减少炎性因子释放,从而治疗CNP。本研究为今后中药制剂治疗CNP提供了新的途径,值得进一步推广。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Effects of Xialiqi Capsules in Regulating the Pyroptosis of Prostate Epithelial Cells Via NLRP3/Caspase-1 Pathway for the Treatment of Chronic Prostatitis.

Chronic prostatitis (CNP) is a prevalent inflammatory disorder among men. The Xialiqi capsule has been reported to alleviate the clinical symptoms of CNP patients, which may be related to its anti-inflammatory effect; yet, its exact mechanism of action remains unclear. In this study, human normal prostate epithelial cells (RWPE-2 cells) were categorized into a control group, a model group, an inhibitor group, along with high, medium, and low drug-containing serum groups (5%, 10%, and 15%, respectively). With the exception of the control group, cell pyroptosis models were created by stimulating with lipopolysaccharide (100 ng/mL) and adenosine triphosphate (5 mM). Subsequently, drug-containing serum and the NOD-like receptor 3 (NLRP3) inhibitor (MCC950) were utilized to intervene with the model cells according to their respective groups. Post-administration of MCC950 and drug-containing serum, an improvement in cell viability was noted in the inhibitor group and medium-high dosage groups (by 20.5%, 38.2%, and 73.2%). Transmission electron microscopy indicated a reduction in the features characteristic of cell pyroptosis. Levels of nitric oxide, interleukin-18 (IL-18), and tumor necrosis factor-α in the cellular supernatant decreased significantly (60.7%, 21.6%, 33.7%, 41.8%; 49.2%, 54.8%, 53.5%, 69.3%; 31.3%, 44.4%, 38.1%, 51.2%). Immunofluorescence showed reduced fluorescence intensity of NLRP3 and Cysteine aspartate protease-1 (Caspase-1) proteins, and Western Blot analysis revealed a significant decline in the expression of NLRP3, pro-Caspase-1, and gasdermin D (20.5%, 45.9%, 58.1%, 74.8%; 23.2%, 36.7%, 51.6%, 51.9%; 15.4%, 28.6%, 33.1%, 39.2%). In vitro experiments suggest that the Xialiqi capsule may treat CNP by regulating prostate epithelial cell pyroptosis and reducing inflammatory factor release via inhibiting the NLRP3/Caspase-1 signaling pathway. This study offers a novel approach for future CNP treatment with traditional Chinese medicine preparations, deserving further promotion.

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来源期刊
American Journal of Men's Health
American Journal of Men's Health PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-
CiteScore
3.70
自引率
4.30%
发文量
107
审稿时长
15 weeks
期刊介绍: American Journal of Men"s Health will be a core resource for cutting-edge information regarding men"s health and illness. The Journal will publish papers from all health, behavioral and social disciplines, including but not limited to medicine, nursing, allied health, public health, health psychology/behavioral medicine, and medical sociology and anthropology.
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