元基因组探索发现了几种参与高氨嗜热沼气工艺中乙酸代谢的新型 "念珠菌 "物种

IF 5.7 2区 生物学
George B. Cheng, Erik Bongcam-Rudloff, Anna Schnürer
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引用次数: 0

摘要

在高氨水平下运行的沼气反应器通常容易受到过程干扰,在嗜热温度下进一步加剧。主要原因被认为是与抑制有关,随后是不同功能微生物群之间的不平衡,集中在厌氧消化的最后两个步骤,涉及醋酸菌,合营养醋酸氧化剂(SAOB)和产甲烷菌。丙酮是反应器效率的关键贡献者,是水解和发酵步骤与最终甲烷生成步骤之间的关键环节。它们的主要产品是醋酸盐,在高氨水平下,进一步由SAOB和氢营养化产甲烷菌转化为沼气。尽管这些功能不同的过程得到了很好的认识,但在高温和氨条件下,对负责任的生物体知之甚少。本研究的主要目的是深入了解在高氨水平(FAN 0.7-1.0 g/L;TAN 3.6-4.4 g/L)。主要目的是鉴定潜在的醋酸和saob。分析了三个反应器的还原乙酰辅酶a途径(Wood-Ljungdahl途径)和甘氨酸合成酶还原酶途径的宏基因组数据。结果显示缺乏真正的醋酸菌,但发现了三种潜在的SAOB候选菌,它们含有WLP,“候选菌热共生菌”、“候选菌热共生菌”、“候选菌热共生菌”和一种潜在的脂质降解菌“候选菌热共生单胞菌”。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Metagenomic Exploration Uncovers Several Novel ‘Candidatus’ Species Involved in Acetate Metabolism in High-Ammonia Thermophilic Biogas Processes

Metagenomic Exploration Uncovers Several Novel ‘Candidatus’ Species Involved in Acetate Metabolism in High-Ammonia Thermophilic Biogas Processes

Biogas reactors operating at elevated ammonia levels are commonly susceptible to process disturbances, further augmented at thermophilic temperatures. The major cause is assumed to be linked to inhibition followed by an imbalance between different functional microbial groups, centred around the last two steps of the anaerobic digestion, involving acetogens, syntrophic acetate oxidisers (SAOB) and methanogens. Acetogens are key contributors to reactor efficiency, acting as the crucial link between the hydrolysis and fermentation steps and the final methanogenesis step. Their major product is acetate, at high ammonia levels further converted by SAOB and hydrogenotrophic methanogens to biogas. Even though these functionally different processes are well recognised, less is known about the responsible organism at elevated temperature and ammonia conditions. The main aim of this study was to garner insights into the penultimate stages in three thermophilic reactors (52°C) operated under high ammonia levels (FAN 0.7–1.0 g/L; TAN 3.6–4.4 g/L). The primary objective was to identify potential acetogens and SAOBs. Metagenomic data from the three reactors were analysed for the reductive acetyl-CoA pathway (Wood–Ljungdahl Pathway) and glycine synthase reductase pathway. The results revealed a lack of true acetogens but uncovered three potential SAOB candidates that harbour the WLP, ‘Candidatus Thermodarwinisyntropha acetovorans’, ‘Candidatus Thermosyntrophaceticus schinkii’, ‘Candidatus Thermotepidanaerobacter aceticum’, and a potential lipid-degrader ‘Candidatus Thermosyntrophomonas ammoiaca’.

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来源期刊
Microbial Biotechnology
Microbial Biotechnology Immunology and Microbiology-Applied Microbiology and Biotechnology
CiteScore
11.20
自引率
3.50%
发文量
162
审稿时长
1 months
期刊介绍: Microbial Biotechnology publishes papers of original research reporting significant advances in any aspect of microbial applications, including, but not limited to biotechnologies related to: Green chemistry; Primary metabolites; Food, beverages and supplements; Secondary metabolites and natural products; Pharmaceuticals; Diagnostics; Agriculture; Bioenergy; Biomining, including oil recovery and processing; Bioremediation; Biopolymers, biomaterials; Bionanotechnology; Biosurfactants and bioemulsifiers; Compatible solutes and bioprotectants; Biosensors, monitoring systems, quantitative microbial risk assessment; Technology development; Protein engineering; Functional genomics; Metabolic engineering; Metabolic design; Systems analysis, modelling; Process engineering; Biologically-based analytical methods; Microbially-based strategies in public health; Microbially-based strategies to influence global processes
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