D. S. Vinogradova, M. S. Bidzhieva, P. S. Kasatsky, V. S. Burdakov, A. A. Grachev, Zh. Yu. Sidorova, A. S. Spitsyna, A. Paleskava, N. A. Verlov, T. A. Shtam, A. L. Konevega
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Control of Conformational Integrity and Aggregation Stability of Monoclonal Antibodies and Their Conjugates by Nanodifferential Scanning Fluorimetry
Therapeutic agents based on monoclonal antibodies (mAbs) are successfully used in the diagnostics and treatment of a wide range of infectious and autoimmune diseases, as well as in the combating malignancies. Due to their high specificity, mAbs are used to target signalling or acting agents to a cell of interest in vivo. Thus, radioactively labelled antibodies are among important tools in nuclear medicine. Stringent control of the conformational integrity and aggregation stability of the mAb preparation is critical for successful biotherapeutic development. In this study, we describe the application of nanodifferential scanning fluorimetry (nanoDSF) to obtain a radioactively labelled monoclonal antibody (161Tb-DOTA-mAb). Using this method, we optimized the procedure of DOTA-mAb conjugate preparation and determined the storage conditions suitable for subsequent rapid radionuclide labelling of the conjugate.
期刊介绍：
Nanobiotechnology Reports publishes interdisciplinary research articles on fundamental aspects of the structure and properties of nanoscale objects and nanomaterials, polymeric and bioorganic molecules, and supramolecular and biohybrid complexes, as well as articles that discuss technologies for their preparation and processing, and practical implementation of products, devices, and nature-like systems based on them. The journal publishes original articles and reviews that meet the highest scientific quality standards in the following areas of science and technology studies: self-organizing structures and nanoassemblies; nanostructures, including nanotubes; functional and structural nanomaterials; polymeric, bioorganic, and hybrid nanomaterials; devices and products based on nanomaterials and nanotechnology; nanobiology and genetics, and omics technologies; nanobiomedicine and nanopharmaceutics; nanoelectronics and neuromorphic computing systems; neurocognitive systems and technologies; nanophotonics; natural science methods in a study of cultural heritage items; metrology, standardization, and monitoring in nanotechnology.
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