水稻内生芽孢杆菌NB茎4的全基因组测序：一种潜在的抑制珍珠粟瘟病的生物防治剂

IF 2.8 3区农林科学 Q2 PLANT SCIENCES

Physiological and Molecular Plant Pathology Pub Date : 2025-03-18 DOI:10.1016/j.pmpp.2025.102663

Kushmitha B , Suhail Ashraf , Nakkeeran S , Johnson I , Saranya N , Mahendra K , Mohammad Raish

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引用次数: 0

摘要

化学农药在可栽培作物的植物病原菌管理中起着至高无上的作用。目前，全球都在强调一种对生态系统影响小、促进植物生长的植物病害管理替代方法。因此，利用耐盐水稻品种诺娜·博克拉的地衣芽孢杆菌对珍珠粟稻瘟病进行了生物防治研究。地衣芽孢杆菌NB茎4基因组注释鉴定为副衣芽孢杆菌NB茎4，编号SRR21528255。基因组分析显示，该菌株共存在958,374,583条reads，平均长度为2906 bp。该基因组共包含11个contigs，总长度为43,13,072 bp，平均G + C含量为45.94%。基因组蛋白编码序列9984个，tRNA区80个，核糖体RNA （r RNA）基因24个，重复区53个。副苔藓芽孢杆菌NB茎4的基因组注释包括1691个假设蛋白和8293个功能定位蛋白。NB茎4基因组中的非核糖体肽合成酶（NRPS）基因簇包括fengycin、putrebactin/ avarfeferrin、butirosin A/butirosin B、lichenysin、streptin、equbactin、bacillibactin和alkylpyrone-407/alkylpyrone-393。副青叶芽孢杆菌NB茎4对珍珠粟稻瘟病菌的体外抑菌活性研究表明，该菌通过产生胡椒苷酮、4-喹啉醇、7-羟基香豆素、创伤酸、生物乙酰丙菊酯、放线菌素、格林霉素、石蜡黄质、尼扎替丁、呋喃酮、曲安奈德、十三烯酸、十六烯酸、二十烯二烯酸、三叶油酸、三叶油酸等抑菌化合物抑制稻瘟病菌菌丝生长。5-羟甲基二氢呋喃-2- 1和环戊二酮。因此，可以探索副拟白僵菌NB茎4在珍珠粟稻瘟病菌防治中的应用。

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Whole genome sequencing of rice endophyte Bacillus paralicheniformis NB stem 4: A potential biocontrol agent for the suppression of pearl millet blast disease

The supreme role in managing the phytopathogens of cultivable crops had been provided by chemical pesticides. An alternative approach for managing the plant disease with less residual effect on the ecosystem and plant growth promoting attributes are emphasized globally. Hence, biocontrol of plant pathogens using Bacillus licheniformis isolated from Nona Bokra, the salt tolerant rice cultivar was explored for the management of blast infecting pearl millet. Genome annotation of Bacillus licheniformis NB stem 4 was identified as B. paralicheniformis NB stem 4 with accession number SRR21528255. Genome analysis revealed the presence of 958, 374, 583 reads with an average (mean) read length of 2906 bp. There were 11 number of contigs in the assembled genome with total length of 43,13,072 bp with an average G + C content of 45.94 %. The protein coding sequences in the genome were 9984, tRNA regions were 80 with 24 ribosomal RNA (r RNA) genes and 53 repeat regions. The genome annotation of B. paralicheniformis NB stem 4 included 1691 hypothetical proteins and 8293 proteins with functional assignments. Non ribosomal peptide synthetase (NRPS) gene clusters in the genome of NB stem 4 include fengycin, putrebactin/avaroferrin, butirosin A/butirosin B, lichenysin, streptin, equibactin, bacillibactin and alkylpyrone-407/alkylpyrone-393. The in vitro screening for examining the antifungal action of B. paralicheniformis NB stem 4 against Magnaporthe grisea of pearl millet suppressed the mycelial growth M. grisea by producing antimicrobial compounds including piperidinone, 4-quinolinol, 7-hydroxycoumarin, traumatic acid, bioallethrin, actinomycin, clindamycin, lycoxanthin, nizatidine, furanone, triamcinolone acetonide, decatrienoic acid, hexadecanoic acid, eicosadienoic acid, trilinolein, 5-hydroxymethyldihydrofuran-2-one and cyclopentanedione. Thus, B. paralicheniformis NB stem 4 can be explored for the management of M. grisea infecting pearl millet.

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来源期刊

Physiological and Molecular Plant Pathology 生物-植物科学

CiteScore

4.30

自引率

7.40%

发文量

130

审稿时长

38 days

期刊介绍： Physiological and Molecular Plant Pathology provides an International forum for original research papers, reviews, and commentaries on all aspects of the molecular biology, biochemistry, physiology, histology and cytology, genetics and evolution of plant-microbe interactions. Papers on all kinds of infective pathogen, including viruses, prokaryotes, fungi, and nematodes, as well as mutualistic organisms such as Rhizobium and mycorrhyzal fungi, are acceptable as long as they have a bearing on the interaction between pathogen and plant.