在大韩民国乌苏里白牙鼩（Crocidura lasiura）中检测和鉴定狼牙病毒

IF 4.1 2区医学 Q1 INFECTIOUS DISEASES

One Health Pub Date : 2025-03-19 DOI:10.1016/j.onehlt.2025.101017

Augustine Natasha , Sarah E. Pye , Kyungmin Park , Shivani Rajoriya , Intae Yang , Jieun Park , Haryo Seno Pangestu , Jongwoo Kim , Yeonsu Oh , Carolina B. López , Jin-Won Song , Won-Keun Kim

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引用次数: 0

摘要

琅琊病毒（LayV）是鼩鼱传播的旁亨尼帕病毒属中唯一记录的人畜共患病原体。在大韩民国发现了其他旁亨尼帕病毒种类，包括加马克病毒和大宁病毒，突出表明该属在该区域的流行。我们回顾性分析了2017年2份乌苏里白牙鼩（Crocidura lasiura）肾脏样本的新一代元基因组测序，并对2023年收集的24份相同物种的肾脏样本进行了副粘病毒筛查。LayV阳性率为12.5%（3 / 24）。随后开发了基于扩增子的测序以获得完整的病毒序列。鉴定出5个朝鲜狼牙病毒（LayV KOR）全基因组：2个来自2017年样本，3个来自2023年样本。LayV KOR在核苷酸和氨基酸水平上分别表现出约80%和95.5%的同源性。系统发育分析强调了LayV - KOR与中国LayV的密切关系。本研究首次在中国以外的鼩鼱中检测到LayV完整序列。

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Detection and characterization of Langya virus in Crocidura lasiura (the Ussuri white-toothed shrew), Republic of Korea

Langya virus (LayV) is the only documented zoonotic agent within the shrew borne Parahenipavirus genus. Other Parahenipavirus species, including Gamak virus and Daeryeong virus, have been discovered in the Republic of Korea, highlighting the prevalence of this genus in the region. We retrospectively analyzed metagenomic next-generation sequencing of two Crocidura lasiura (the Ussuri white-toothed shrew) kidney samples from 2017, followed by paramyxovirus screening of 24 kidney samples from the same species collected in 2023. The LayV positivity rate was 12.5 % (3 of 24). Amplicon-based sequencing was subsequently developed to obtain the complete viral sequences. Five complete genomes of Langya virus Korea (LayV KOR) were identified: two from 2017 samples and three from 2023 samples. LayV KOR exhibited approximately 80 % and 95.5 % homology at the nucleotide and amino acid levels, respectively. Phylogenetic analysis underscored the close relationship between LayV KOR and LayV from China. This study represents the first detection of LayV complete sequences in shrews outside of China.

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来源期刊

One Health Medicine-Infectious Diseases

CiteScore

8.10

自引率

4.00%

发文量

审稿时长

18 weeks

期刊介绍： One Health - a Gold Open Access journal. The mission of One Health is to provide a platform for rapid communication of high quality scientific knowledge on inter- and intra-species pathogen transmission, bringing together leading experts in virology, bacteriology, parasitology, mycology, vectors and vector-borne diseases, tropical health, veterinary sciences, pathology, immunology, food safety, mathematical modelling, epidemiology, public health research and emergency preparedness. As a Gold Open Access journal, a fee is payable on acceptance of the paper. Please see the Guide for Authors for more information. Submissions to the following categories are welcome: Virology, Bacteriology, Parasitology, Mycology, Vectors and vector-borne diseases, Co-infections and co-morbidities, Disease spatial surveillance, Modelling, Tropical Health, Discovery, Ecosystem Health, Public Health.