鞣花酸通过保护线粒体功能免受活性氧损伤来维持解冻后山羊精子质量

IF 2.1 3区生物学 Q2 BIOLOGY

Cryobiology Pub Date : 2025-03-24 DOI:10.1016/j.cryobiol.2025.105231

Zhendong Zhu , Wenjia Li , Kexin Ding , Eslam M. Bastawy , Ahmed Mohamed Kamel , Xin Kou , Lingjiang Min

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引用次数: 0

摘要

本研究旨在探讨抗氧化剂鞣花酸（EA）在冷冻和解冻后对山羊精子质量的影响。用含有0、1.25、2.5、5和10 μM EA的三柠檬酸-葡萄糖（TCG）填充剂冷冻山羊精液，蛋黄占最终浓度的20% (v/v)，甘油占最终浓度的5% （v/v）。评估山羊精子解冻后活力、顶体完整性、质膜完整性、线粒体活性、ATP含量、NADH/NAD+水平和NADH- coq活性。此外，为了阐明EA如何增强山羊精子的特性，我们还检测了解冻后精子线粒体活性氧（ROS）水平、丙二醛（MDA）水平、DNA氧化损伤、细胞凋亡、NADH脱氢酶1 （MT-ND1）和NADH脱氢酶6 （MT-ND6）蛋白水平以及4-羟基壬烯醛（4-HNE）水平。结果表明，与其他浓度（0 μM、1.25 μM、2.5 μM、5 μM和10 μM）相比，5 μM EA处理组的细胞活力、质膜完整性和顶体完整性率均有所提高。此外，与所有其他治疗组相比，5 μM EA组线粒体活性和ATP含量显著提高，ROS和MDA水平显著降低。补充EA可降低精子4-HNE水平和氧化DNA损伤。此外，发现EA （5 μM）显著(p <；0.05)降低精子凋亡水平。此外，添加5 μM EA维持解冻后精子MT-ND1和MT-ND6水平，降低ROS对MT-ND1和MT-ND6的负面影响，从而维持线粒体生成ATP的功能。这些结果表明，添加鞣花酸可以通过减少ROS损伤和维持线粒体ATP生成功能来维持山羊解冻后精子质量。抗氧化处理，如鞣花酸是维持冷冻解冻精子质量的有用工具。

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Ellagic acid maintains post-thaw goat sperm quality via protecting mitochondrial function from ROS damage

This study aimed to investigate the effects of ellagic acid (EA), an antioxidant, on goat sperm quality after freezing and thawing. Goat semen was frozen using Tris-citric acid-glucose (TCG) extender containing 0, 1.25, 2.5, 5, and 10 μM of EA. Egg yolk represented 20 % (v/v) and glycerol represented 5 % (v/v) of the extender's final concentration. Goat sperm post-thaw motility, acrosome integrity, plasma membrane integrity, mitochondrial activity, ATP content, NADH/NAD⁺ levels, and NADH-CoQ activity were evaluated. Moreover, to elucidate how EA enhanced the goat sperm characteristics, the post-thaw sperm mitochondrial reactive oxygen species (ROS) level, malondialdehyde (MDA) level, oxidative DNA damage, apoptosis, levels of NADH dehydrogenase 1 (MT-ND1) and NADH dehydrogenase 6 (MT-ND6) proteins, and the 4-hydroxynonenal (4-HNE) level were also measured after thawing. The results demonstrated that motility, plasma membrane integrity, and acrosome integrity rates were enhanced in the group treated with 5 μM of EA compared to the other concentrations (0 μM, 1.25 μM, 2.5 μM, 5, and 10 μM). Moreover, mitochondrial activity and ATP content were notably superior in the 5 μM EA group compared to all other treatment groups, along with a considerable decrease in ROS and MDA levels. The 4-HNE level and oxidative DNA damage in sperm were also reduced by EA supplementation. Additionally, it was found that EA (5 μM) significantly (p < 0.05) decreased sperm apoptosis levels. Furthermore, the addition of 5 μM EA maintained the post-thaw sperm MT-ND1 and MT-ND6 levels and reduced the negative impact of ROS on MT-ND1 and MT-ND6, thereby sustaining mitochondrial function for ATP generation. These results suggest that ellagic acid supplementation could maintain goat post-thaw sperm quality by reducing ROS damage and maintaining mitochondrial function for ATP generation. Antioxidant treatments, such as ellagic acid are a useful tool for maintaining frozen-thawed sperm quality.

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来源期刊

Cryobiology 生物-生理学

CiteScore

5.40

自引率

7.40%

发文量

审稿时长

56 days

期刊介绍： Cryobiology: International Journal of Low Temperature Biology and Medicine publishes research articles on all aspects of low temperature biology and medicine. Research Areas include: • Cryoprotective additives and their pharmacological actions • Cryosurgery • Freeze-drying • Freezing • Frost hardiness in plants • Hibernation • Hypothermia • Medical applications of reduced temperature • Perfusion of organs • All pertinent methodologies Cryobiology is the official journal of the Society for Cryobiology.