Dynamic hydrogels for biofabrication: A review

Reversibly crosslinked dynamic hydrogels have emerged as a significant material platform for biomedical applications owing to their distinctive time-dependent characteristics, including shear-thinning, self-healing, stress relaxation, and creep. These physical properties permit the use of dynamic hydrogels as injectable carriers or three-dimensional printable bioinks. It is noteworthy that matrix dynamics can serve as physical cues that stimulate cellular processes. Therefore, dynamic hydrogels are preferred for tissue engineering and biofabrication, which seek to create functional tissue constructs that require regulation of cellular processes. This review summarizes the critical biophysical properties of dynamic hydrogels, various cellular processes and related mechanisms triggered by hydrogel dynamics, particularly in three-dimensional culture scenarios. Subsequently, we present an overview of advanced biofabrication techniques, particularly 3D bioprinting, of dynamic hydrogels for the large-scale production of tissue and organ engineering models. This review presents an overview of the strategies that can be used to expand the range of applications of dynamic hydrogels in biofabrication, while also addressing the challenges and opportunities that arise in the field. This review highlights the importance of matrix dynamics in regulating cellular processes and elucidates strategies for leveraging them in the context of biofabrication.