自由基s -腺苷蛋氨酸（SAM）酶扩大核糖体合成和翻译后修饰肽（RiPP）天然产物的分子多样性：最新进展和机制见解

IF 4 2区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Chinese Journal of Natural Medicines Pub Date : 2025-03-01 DOI:10.1016/S1875-5364(25)60845-4

Jiawei Feng, Jiarong Mo, Xinya Hemu

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引用次数: 0

摘要

核糖体合成和翻译后修饰肽（RiPPs）构成了一个庞大而多样的生物活性肽家族。这些肽由核糖体合成，然后由各种剪裁酶修饰，具有广泛的化学空间。在这些修饰中，自由基s -腺苷甲硫氨酸（rSAM）酶利用独特的自由基化学引入多种新的肽结构，这对其活性至关重要。本文综述了由rSAM酶催化的RiPPs的主要修饰类型，并结合了蛋白质结构分析技术和计算方法的最新进展。此外，通过对最新晶体结构的分析，阐明了这些酶的不同催化机制和底物选择性。

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Expanding molecular diversity of ribosomally synthesized and post-translationally modified peptide (RiPP) natural products by radical S-adenosylmethionine (SAM) enzymes: recent advances and mechanistic insights

Ribosomally synthesized and post-translationally modified peptides (RiPPs) constitute a vast and diverse family of bioactive peptides. These peptides, synthesized by ribosomes and subsequently modified by various tailoring enzymes, possess a wide chemical space. Among these modifications, radical S-adenosylmethionine (rSAM) enzymes employ unique radical chemistry to introduce a variety of novel peptide structures, which are crucial for their activity. This review examines the major types of modifications in RiPPs catalyzed by rSAM enzymes, incorporating recent advancements in protein structure analysis techniques and computational methods. Additionally, it elucidates the diverse catalytic mechanisms and substrate selectivity of these enzymes through an analysis of the latest crystal structures.

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来源期刊

Chinese Journal of Natural Medicines INTEGRATIVE & COMPLEMENTARY MEDICINE-PHARMACOLOGY & PHARMACY

CiteScore

7.50

自引率

4.30%

发文量

2235

期刊介绍： The Chinese Journal of Natural Medicines (CJNM), founded and sponsored in May 2003 by China Pharmaceutical University and the Chinese Pharmaceutical Association, is devoted to communication among pharmaceutical and medical scientists interested in the advancement of Traditional Chinese Medicines (TCM). CJNM publishes articles relating to a broad spectrum of bioactive natural products, leading compounds and medicines derived from Traditional Chinese Medicines (TCM). Topics covered by the journal are: Resources of Traditional Chinese Medicines; Interaction and complexity of prescription; Natural Products Chemistry (including structure modification, semi-and total synthesis, bio-transformation); Pharmacology of natural products and prescription (including pharmacokinetics and toxicology); Pharmaceutics and Analytical Methods of natural products.