黄酮类芦丁有效治疗口腔微生物感染及生物膜的体外研究

Q1 Medicine
K.P. Sowmya , M. Surenthar , V. Lekha Sree
{"title":"黄酮类芦丁有效治疗口腔微生物感染及生物膜的体外研究","authors":"K.P. Sowmya ,&nbsp;M. Surenthar ,&nbsp;V. Lekha Sree","doi":"10.1016/j.jobcr.2025.03.007","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>Oral microbial infections and biofilm-associated conditions, such as dental caries, gingivitis, and periodontitis, remain major challenges in oral healthcare. Biofilms provide resistance to conventional antimicrobial treatments, necessitating innovative solutions. This study investigates rutin, a flavonoid glycoside, for its antimicrobial and antibiofilm properties against key oral pathogens.</div></div><div><h3>Aim</h3><div>To evaluate the antibacterial and antibiofilm efficacy of rutin against oral pathogens, including <em>Streptococcus mutans</em>, <em>Pseudomonas aeruginosa</em>, and <em>Candida albicans</em>, and assess its hemocompatibility as a safe therapeutic agent.</div></div><div><h3>Methodology</h3><div>Oral pathogens were isolated from clinical samples and identified using the VITEK®2 Compact System. Antimicrobial activity of five flavonoids was screened using the well diffusion method, with rutin demonstrating the highest efficacy. Minimum inhibitory concentration (MIC) and antibiofilm activity were assessed using broth microdilution and crystal violet staining, respectively. Confocal Laser Scanning Microscopy analyzed live/dead cells in treated biofilms. Hemocompatibility was evaluated via a hemolysis assay.</div></div><div><h3>Results</h3><div>Rutin exhibited significant antimicrobial activity with zone of inhibition as 19 mm (<em>C. albicans</em>), 17 mm (<em>P. aeruginosa</em>), and 17 mm (<em>S. mutans</em>). MIC against mixed biofilms was 10 mM, while treatment at 2 × MIC reduced biofilm biomass by 92 %. Live/dead analysis confirmed extensive microbial cell death in biofilms. Hemolysis rates below 5 % established rutin's biocompatibility.</div></div><div><h3>Conclusion</h3><div>Rutin demonstrates potent antimicrobial and antibiofilm efficacy with excellent safety, suggesting its potential as an alternative therapy for biofilm-associated oral infections. Further in vivo studies are warranted to validate these findings.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"15 3","pages":"Pages 541-547"},"PeriodicalIF":0.0000,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effective treatment of oral microbial infections and biofilm using flavonoid rutin - An in vitro study\",\"authors\":\"K.P. Sowmya ,&nbsp;M. Surenthar ,&nbsp;V. Lekha Sree\",\"doi\":\"10.1016/j.jobcr.2025.03.007\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>Oral microbial infections and biofilm-associated conditions, such as dental caries, gingivitis, and periodontitis, remain major challenges in oral healthcare. Biofilms provide resistance to conventional antimicrobial treatments, necessitating innovative solutions. This study investigates rutin, a flavonoid glycoside, for its antimicrobial and antibiofilm properties against key oral pathogens.</div></div><div><h3>Aim</h3><div>To evaluate the antibacterial and antibiofilm efficacy of rutin against oral pathogens, including <em>Streptococcus mutans</em>, <em>Pseudomonas aeruginosa</em>, and <em>Candida albicans</em>, and assess its hemocompatibility as a safe therapeutic agent.</div></div><div><h3>Methodology</h3><div>Oral pathogens were isolated from clinical samples and identified using the VITEK®2 Compact System. Antimicrobial activity of five flavonoids was screened using the well diffusion method, with rutin demonstrating the highest efficacy. Minimum inhibitory concentration (MIC) and antibiofilm activity were assessed using broth microdilution and crystal violet staining, respectively. Confocal Laser Scanning Microscopy analyzed live/dead cells in treated biofilms. Hemocompatibility was evaluated via a hemolysis assay.</div></div><div><h3>Results</h3><div>Rutin exhibited significant antimicrobial activity with zone of inhibition as 19 mm (<em>C. albicans</em>), 17 mm (<em>P. aeruginosa</em>), and 17 mm (<em>S. mutans</em>). MIC against mixed biofilms was 10 mM, while treatment at 2 × MIC reduced biofilm biomass by 92 %. Live/dead analysis confirmed extensive microbial cell death in biofilms. Hemolysis rates below 5 % established rutin's biocompatibility.</div></div><div><h3>Conclusion</h3><div>Rutin demonstrates potent antimicrobial and antibiofilm efficacy with excellent safety, suggesting its potential as an alternative therapy for biofilm-associated oral infections. Further in vivo studies are warranted to validate these findings.</div></div>\",\"PeriodicalId\":16609,\"journal\":{\"name\":\"Journal of oral biology and craniofacial research\",\"volume\":\"15 3\",\"pages\":\"Pages 541-547\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-03-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of oral biology and craniofacial research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2212426825000703\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of oral biology and craniofacial research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212426825000703","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0

摘要

口腔微生物感染和生物膜相关疾病,如龋齿、牙龈炎和牙周炎,仍然是口腔保健的主要挑战。生物膜对传统的抗菌素治疗具有耐药性,因此需要创新的解决方案。本研究研究了芦丁(一种黄酮类苷)对主要口腔病原体的抗菌和抗生物膜特性。目的评价芦丁对变形链球菌、铜绿假单胞菌、白色念珠菌等口腔病原菌的抗菌和抗生物膜作用,并评价其作为一种安全的治疗药物的血液相容性。方法从临床样品中分离病原菌,使用VITEK®2 Compact System进行鉴定。采用孔扩散法对5种黄酮类化合物进行抑菌活性筛选,以芦丁的抑菌活性最高。最低抑菌浓度(MIC)和抗菌膜活性分别用微肉汤稀释法和结晶紫染色法测定。共聚焦激光扫描显微镜分析处理过的生物膜中的活细胞和死细胞。通过溶血试验评估血液相容性。结果芦丁对白色念珠菌的抑制区为19 mm,对铜绿假单胞菌的抑制区为17 mm,对变形链球菌的抑制区为17 mm。混合生物膜的MIC为10 mM,而2倍MIC处理使生物膜生物量减少92%。活/死分析证实生物膜中大量微生物细胞死亡。溶血率低于5%确立了芦丁的生物相容性。结论芦丁具有较强的抗菌和抗生物膜作用,安全性好,有望成为生物膜相关性口腔感染的替代治疗药物。需要进一步的体内研究来验证这些发现。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Effective treatment of oral microbial infections and biofilm using flavonoid rutin - An in vitro study

Effective treatment of oral microbial infections and biofilm using flavonoid rutin - An in vitro study

Background

Oral microbial infections and biofilm-associated conditions, such as dental caries, gingivitis, and periodontitis, remain major challenges in oral healthcare. Biofilms provide resistance to conventional antimicrobial treatments, necessitating innovative solutions. This study investigates rutin, a flavonoid glycoside, for its antimicrobial and antibiofilm properties against key oral pathogens.

Aim

To evaluate the antibacterial and antibiofilm efficacy of rutin against oral pathogens, including Streptococcus mutans, Pseudomonas aeruginosa, and Candida albicans, and assess its hemocompatibility as a safe therapeutic agent.

Methodology

Oral pathogens were isolated from clinical samples and identified using the VITEK®2 Compact System. Antimicrobial activity of five flavonoids was screened using the well diffusion method, with rutin demonstrating the highest efficacy. Minimum inhibitory concentration (MIC) and antibiofilm activity were assessed using broth microdilution and crystal violet staining, respectively. Confocal Laser Scanning Microscopy analyzed live/dead cells in treated biofilms. Hemocompatibility was evaluated via a hemolysis assay.

Results

Rutin exhibited significant antimicrobial activity with zone of inhibition as 19 mm (C. albicans), 17 mm (P. aeruginosa), and 17 mm (S. mutans). MIC against mixed biofilms was 10 mM, while treatment at 2 × MIC reduced biofilm biomass by 92 %. Live/dead analysis confirmed extensive microbial cell death in biofilms. Hemolysis rates below 5 % established rutin's biocompatibility.

Conclusion

Rutin demonstrates potent antimicrobial and antibiofilm efficacy with excellent safety, suggesting its potential as an alternative therapy for biofilm-associated oral infections. Further in vivo studies are warranted to validate these findings.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
4.90
自引率
0.00%
发文量
133
审稿时长
167 days
期刊介绍: Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信