恼怒榕叶乙酸乙酯提取物提取物(FE1)的植物化学成分、抗氧化活性及抗菌作用

Joy Cecilia Atawodi , Jonathan Ilemona Achika , Sunday Ene-Ojo Atawodi , Rachael Gbekele-Oluwa Ayo
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引用次数: 0

摘要

微生物耐药性和氧化应激相关疾病的日益流行促使人们寻找具有治疗潜力的天然化合物。传统医学中常用的无花果,被认为具有值得研究的生物活性。本研究旨在鉴定恼怒榕叶提取物的化学成分,并评价其抗氧化和抗菌性能,重点研究一个关键的乙酸乙酯衍生部位(FE1)。用甲醇、乙酸乙酯、氯仿和己烷对恼怒榕叶进行萃取。从甲醇提取物中分离得到FE1组分,利用UV、FT-IR和GC-MS技术对其进行了表征。通过DPPH自由基清除和铁离子还原能力测定来评估抗氧化活性。采用琼脂孔扩散法对大肠杆菌、奇异变形杆菌和热带假丝酵母进行抑菌效果试验。FE1的紫外和红外光谱分析鉴定出羟基和羰基,表明存在生物活性脂肪酸。GC-MS证实其主要成分为十六烷酸(棕榈酸)、壬烷和2-戊烷酮。FE1表现出中等的抗氧化活性(IC50 = 87.623 ± 0.65 μg/mL)和较强的抗菌作用,特别是对大肠杆菌(35 mm)、P. mirabilis(30 mm)和C. tropicalis(25 mm)。从无花果中提取的FE1具有显著的抗菌和抗氧化潜力,是一种有前景的新型治疗药物。进一步的研究应探讨其作用机制及其在联合治疗中的应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Phytochemical composition, antioxidant activity and antimicrobial efficacy of ethyl acetate extract-derived Fraction (FE1) from Ficus exasperata Vahl Leaf
The increasing prevalence of microbial resistance and oxidative stress-related diseases has driven the search for natural compounds with therapeutic potential. Ficus exasperata Vahl, commonly used in traditional medicine, is believed to possess bioactive properties worth investigating. This study aimed to identify the chemical constituents of Ficus exasperata leaf extract and evaluate its antioxidant and antimicrobial properties, focusing on a key ethyl acetate-derived fraction (FE1). Ficus exasperata leaves were sequentially extracted using methanol, ethyl acetate, chloroform, and hexane. Fraction FE1, isolated from the methanolic extract, was characterized using UV, FT-IR, and GC-MS techniques. Antioxidant activity was assessed using DPPH radical scavenging and ferric-ion-reducing power assays. Antimicrobial efficacy was tested against Escherichia coli, Proteus mirabilis, and Candida tropicalis using the agar well diffusion method. The UV and FT-IR analyses of FE1 identified hydroxyl and carbonyl groups, suggesting the presence of bioactive fatty acids. GC-MS confirmed hexadecanoic acid (palmitic acid) as the major component, along with nonadecane and 2-pentadecanone. The FE1 exhibited moderate antioxidant activity (IC50 = 87.623 ± 0.65 μg/mL) and strong antimicrobial effects, particularly against E. coli (35 mm), P. mirabilis (30 mm), and C. tropicalis (25 mm). The FE1 from Ficus exasperata shows significant antimicrobial and antioxidant potential, making it a promising candidate for novel therapeutic development. Further research should explore its mechanisms of action and possible applications in combination therapies.
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