罗勒乙醇提取物的抑菌活性及其对黑腹果蝇的毒性研究

Carlos Alonso Leite dos Santos , Raimundo Luiz da Silva Pereira , Luís Pereira-de-Morais , George Joaquim Garcia Santos , Henrique Douglas Melo Coutinho , Francisco Assis Bezerra da Cunha , Fabiola Fernandes Galvão Rodrigues , Dárcio Luiz de Sousa Júnior , Julio Cesar Silva
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引用次数: 0

摘要

致力于研究药用植物的抗菌活性的研究包括各种各样的物种,植物提取物的使用正在成为一种高度相关的疾病预防替代方案。在这种情况下,通常被称为罗勒的Ocimum gratissimum L.因其显著的抗菌性能而被广泛研究。在这项特殊的研究中,我们评估了虎骨草乙醇提取物(EEOg)的抗菌活性及其与抗生素氨苄西林、阿米卡星、诺氟沙星和青霉素的相互作用。我们的研究包括用肉汤微量稀释技术来确定最低抑制浓度,分析与抗生素的联合活性,以及评估食物中添加的EEOg,以评估暴露48 小时后对果蝇模型死亡率和地向性的影响。结果表明,与抗生素阿米卡星联合使用时,大肠杆菌和铜绿假单胞菌对该提取物有明显的敏感性,浓度为25 mg/mL时毒性较低。这些发现表明这种提取物在药物开发中用于治疗由这些细菌引起的感染的潜在用途。然而,需要进一步的研究来阐明其潜在的机制,并确保该提取物在治疗应用中的安全性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antibacterial activity of ethanolic extract of Ocimum gratissimum L. (BASIL) and its toxicity against Drosophila melanogaster
Research dedicated to investigating the antimicrobial activity of medicinal plants encompasses a variety of species, with the use of plant extracts emerging as a highly relevant alternative for disease prevention. In this context, Ocimum gratissimum L., commonly known as basil, has been widely studied for its remarkable antibacterial properties. In this specific study, we evaluated the antibacterial activity of the ethanolic extract of Ocimum gratissimum L. (EEOg) and its interaction with the antibiotics ampicillin, amikacin, norfloxacin, and penicillin. Our research included the broth microdilution technique for determining the minimum inhibitory concentration, analysis of combined activity with antibiotics, and assessment of EEOg supplementation in food to evaluate the impact on mortality and geotaxis in the Drosophila melanogaster model after 48 hours of exposure. The results showed that Escherichia coli and Pseudomonas aeruginosa exhibited notable sensitivity to the extract when combined with the antibiotic amikacin, with low toxicity observed at a concentration of 25 mg/mL. These findings indicate the potential use of this extract in drug development for treating infections caused by these bacteria. However, further studies are necessary to elucidate the underlying mechanisms and ensure the safety of this extract in therapeutic applications.
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