噬菌体燃料防御系统

IF 12.9 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nature chemical biology Pub Date : 2025-03-24 DOI:10.1038/s41589-025-01875-7

Yiyun Song

引用次数: 0

摘要

在噬菌体感染的反应中，细菌细胞产生环核苷酸或环adp核糖作为第二信使，激活下游效应蛋白，诱导细胞死亡或直接抑制噬菌体复制。然而，尚不清楚其他分子是否也起到细菌免疫信号分子的作用。现在，Zeng, Hu, Zhao， Rao等人在大肠杆菌中发现了一种抗噬菌体防御系统，该系统产生脱氧肌苷5 ' -三磷酸（dITP）作为第二信使分子。该团队通过比较基因组分析确定了这个系统，并通过遗传和生化特征进行了验证。该系统由3个基因（komA、komB和komC）组成，分别编码腺苷脱氨酶（komA）、ddip结合蛋白（komB）和NADase （komC）。研究小组重建了dITP的生物合成途径，揭示了KomA与噬菌体编码的dNMP激酶（DNK）和宿主的dNDP激酶（NDK）合作，将dAMP转化为dITP。编码DNK基因突变的噬菌体对这种三基因免疫系统有抵抗力。在dITP结合后，KomB和KomC形成一个效应复合物，降解NAD+（细菌细胞必需的代谢辅助因子），导致被感染细胞从群体中消除。考虑到该系统利用噬菌体自身的DNK酶来启动免疫反应，研究人员将其命名为孔明，以向以战术智慧闻名的中国古代战略家孔明致敬。

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来源期刊

Nature chemical biology 生物-生化与分子生物学

CiteScore

23.90

自引率

1.40%

发文量

238

审稿时长

12 months

期刊介绍： Nature Chemical Biology stands as an esteemed international monthly journal, offering a prominent platform for the chemical biology community to showcase top-tier original research and commentary. Operating at the crossroads of chemistry, biology, and related disciplines, chemical biology utilizes scientific ideas and approaches to comprehend and manipulate biological systems with molecular precision. The journal embraces contributions from the growing community of chemical biologists, encompassing insights from chemists applying principles and tools to biological inquiries and biologists striving to comprehend and control molecular-level biological processes. We prioritize studies unveiling significant conceptual or practical advancements in areas where chemistry and biology intersect, emphasizing basic research, especially those reporting novel chemical or biological tools and offering profound molecular-level insights into underlying biological mechanisms. Nature Chemical Biology also welcomes manuscripts describing applied molecular studies at the chemistry-biology interface due to the broad utility of chemical biology approaches in manipulating or engineering biological systems. Irrespective of scientific focus, we actively seek submissions that creatively blend chemistry and biology, particularly those providing substantial conceptual or methodological breakthroughs with the potential to open innovative research avenues. The journal maintains a robust and impartial review process, emphasizing thorough chemical and biological characterization.