两种锌指同源核转录因子 OsZHD1 和 OsZHD2 通过影响水稻细胞增殖冗余地控制粒度

IF 4.8 1区 农林科学 Q1 AGRONOMY
Rice Pub Date : 2025-03-22 DOI:10.1186/s12284-025-00774-8
Mingliang Guo, Chun Zheng, Chao Shi, Xiaozhuan Lu, Zeyuan She, Shuyu Jiang, Dagang Tian, Yuan Qin
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引用次数: 0

摘要

籽粒大小是决定籽粒产量和品质的重要因素,籽粒大小由籽粒的三维结构(长、宽、厚)决定。锌指同源结构域(ZINC FINGER-HOMEODOMAIN, ZHD)蛋白在植物的生长发育中起着至关重要的作用。然而,关于ZHD蛋白在生殖发育中的功能的信息很少。在这里,我们深入表征了oszhd1, oszhd2和oszhd10oszhd2的表型。OsZHD1/2的单突变体与野生型相似。然而,双突变体表现为侏儒症,生殖器官较小,晶粒尺寸更短、更窄、更薄。Oszhd1oszhd2外薄壁细胞总长度、总数量和单细胞宽度显著减少;与野生型、oszhd1-1、oszhd2-1相比,oszhd1ozhd2的纵表皮细胞平均宽度减小,但内颖片和外颖片的长度增加。OsZHD1和OsZHD2编码核蛋白,主要分布在茎和发育中的小穗中,对籽粒大小有一定的影响。同时,酵母双杂交、双分子荧光互补和共免疫沉淀实验表明,OsZHD1可以直接与OsZHD2相互作用。差异表达分析显示,与野生型和单突变体相比,oszhd1oszhd2中表达下调的839个基因主要富集于次生代谢物生物合成、膜整体组分和转运蛋白活性途径。此外,在RNA-seq数据中观察到细胞周期和扩增相关基因以及颗粒大小相关基因的表达改变是可靠的,与qRT-PCR结果高度一致。综上所述,我们的研究结果表明,OsZHD1/2具有功能冗余,并通过影响水稻细胞增殖来调节晶粒大小。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The OsZHD1 and OsZHD2, Two Zinc Finger Homeobox Transcription Factor, Redundantly Control Grain Size by Influencing Cell Proliferation in Rice.

Grain size is vital determinant for grain yield and quality, which specified by its three-dimensional structure of seeds (length, width and thickness). The ZINC FINGER-HOMEODOMAIN (ZHD) proteins play critical roles in plant growth and development. However, the information regarding the function in reproductive development of ZHD proteins is scarce. Here, we deeply characterized the phenotype of oszhd1, oszhd2, and oszhd1oszhd2. The single mutants of OsZHD1/2 were similar with wild type. Nevertheless, the double mutant displayed dwarfism and smaller reproductive organs, and shorter, narrower, and thinner grain size. oszhd1oszhd2 revealed a significant decrease in total cell length and number, and single cell width in outer parenchyma; reducing the average width of longitudinal epidermal cells, but the length were increased in outer and inner glumes of oszhd1oszhd2 compared with wild-type, oszhd1-1, oszhd2-1, respectively. OsZHD1 and OsZHD2 encoded the nucleus protein and were distributed predominately in stem and the developing spikelets, asserting their roles in grain size. Meanwhile, yeast two-hybrid, bimolecular fluorescence complementation, and Co-immunoprecipitation assay clarified that OsZHD1 could directly interacted with OsZHD2. The differential expression analysis showed that 839 DEGs, which were down-regulated in oszhd1oszhd2 than wild type and single mutants, were mainly enriched in secondary metabolite biosynthetic, integral component of membrane, and transporter activity pathway. Moreover, it is reliable that the altered expression of cell cycle and expansion-related and grain size-related genes were observed in RNA-seq data, highly consistent with the qRT-PCR results. Altogether, our results suggest that OsZHD1/2 are functional redundancy and involved in regulating grain size by influencing cell proliferation in rice.

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来源期刊
Rice
Rice AGRONOMY-
CiteScore
10.10
自引率
3.60%
发文量
60
审稿时长
>12 weeks
期刊介绍: Rice aims to fill a glaring void in basic and applied plant science journal publishing. This journal is the world''s only high-quality serial publication for reporting current advances in rice genetics, structural and functional genomics, comparative genomics, molecular biology and physiology, molecular breeding and comparative biology. Rice welcomes review articles and original papers in all of the aforementioned areas and serves as the primary source of newly published information for researchers and students in rice and related research.
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