{"title":"kr<s:1> ppel同源物1是调节白纹伊蚊（Skuse）（双翅目：库蚊科）繁殖发育的一种幼体激素转录因子。","authors":"Ya-Hui Chen, Cheng Wu, Yu-Yang Xie, Yan-Hui Zhang, Xi-Tong Huang, Fen Hu, Li-Hua Xie","doi":"10.1007/s00436-025-08479-z","DOIUrl":null,"url":null,"abstract":"<p><p>The control of Aedes albopictus (Skuse) (Diptera: Culicidae), a vector for several important viral diseases, is crucial for mitigating mosquito-borne diseases. This study focused on the Krüppel homolog 1 (Kr-h1) gene, a transcription factor in juvenile hormone (JH) signaling, which plays a pivotal role in inhibiting metamorphosis and promoting adult reproduction. We characterized Aedes albopictus Kr-h1(AalbKr-h1), identified its eight zinc finger domains, and confirmed its orthology among insects through phylogenetic analysis. Expression profiling across life stages showed high level of expression in eggs, late larvae, and adults, with minimal expression in pupae. In adults, AalbKr-h1 was most active in the fat body and ovaries. Exposing larvae to a JH analogue significantly upregulated AalbKr-h1 expression in both larval and adult stages. RNAi-mediated knockdown of Kr-h1 protein reduced egg reproduction, survival rate and gene expression levels. These results provide a solid foundation for further exploration of the function of the AalbKr-h1 gene and the potential development of novel strategies for mosquito control and prevention of mosquito-borne diseases.</p>","PeriodicalId":19968,"journal":{"name":"Parasitology Research","volume":"124 3","pages":"36"},"PeriodicalIF":1.8000,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11928384/pdf/","citationCount":"0","resultStr":"{\"title\":\"Krüppel homolog 1, a juvenile hormone transcription factor, regulates the reproduction and development of Aedes albopictus(Skuse) (Diptera: Culicidae).\",\"authors\":\"Ya-Hui Chen, Cheng Wu, Yu-Yang Xie, Yan-Hui Zhang, Xi-Tong Huang, Fen Hu, Li-Hua Xie\",\"doi\":\"10.1007/s00436-025-08479-z\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The control of Aedes albopictus (Skuse) (Diptera: Culicidae), a vector for several important viral diseases, is crucial for mitigating mosquito-borne diseases. This study focused on the Krüppel homolog 1 (Kr-h1) gene, a transcription factor in juvenile hormone (JH) signaling, which plays a pivotal role in inhibiting metamorphosis and promoting adult reproduction. We characterized Aedes albopictus Kr-h1(AalbKr-h1), identified its eight zinc finger domains, and confirmed its orthology among insects through phylogenetic analysis. Expression profiling across life stages showed high level of expression in eggs, late larvae, and adults, with minimal expression in pupae. In adults, AalbKr-h1 was most active in the fat body and ovaries. Exposing larvae to a JH analogue significantly upregulated AalbKr-h1 expression in both larval and adult stages. RNAi-mediated knockdown of Kr-h1 protein reduced egg reproduction, survival rate and gene expression levels. These results provide a solid foundation for further exploration of the function of the AalbKr-h1 gene and the potential development of novel strategies for mosquito control and prevention of mosquito-borne diseases.</p>\",\"PeriodicalId\":19968,\"journal\":{\"name\":\"Parasitology Research\",\"volume\":\"124 3\",\"pages\":\"36\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2025-03-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11928384/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Parasitology Research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s00436-025-08479-z\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Parasitology Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s00436-025-08479-z","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Krüppel homolog 1, a juvenile hormone transcription factor, regulates the reproduction and development of Aedes albopictus(Skuse) (Diptera: Culicidae).
The control of Aedes albopictus (Skuse) (Diptera: Culicidae), a vector for several important viral diseases, is crucial for mitigating mosquito-borne diseases. This study focused on the Krüppel homolog 1 (Kr-h1) gene, a transcription factor in juvenile hormone (JH) signaling, which plays a pivotal role in inhibiting metamorphosis and promoting adult reproduction. We characterized Aedes albopictus Kr-h1(AalbKr-h1), identified its eight zinc finger domains, and confirmed its orthology among insects through phylogenetic analysis. Expression profiling across life stages showed high level of expression in eggs, late larvae, and adults, with minimal expression in pupae. In adults, AalbKr-h1 was most active in the fat body and ovaries. Exposing larvae to a JH analogue significantly upregulated AalbKr-h1 expression in both larval and adult stages. RNAi-mediated knockdown of Kr-h1 protein reduced egg reproduction, survival rate and gene expression levels. These results provide a solid foundation for further exploration of the function of the AalbKr-h1 gene and the potential development of novel strategies for mosquito control and prevention of mosquito-borne diseases.
期刊介绍：
The journal Parasitology Research covers the latest developments in parasitology across a variety of disciplines, including biology, medicine and veterinary medicine. Among many topics discussed are chemotherapy and control of parasitic disease, and the relationship of host and parasite.
Other coverage includes: Protozoology, Helminthology, Entomology; Morphology (incl. Pathomorphology, Ultrastructure); Biochemistry, Physiology including Pathophysiology;
Parasite-Host-Relationships including Immunology and Host Specificity; life history, ecology and epidemiology; and Diagnosis, Chemotherapy and Control of Parasitic Diseases.
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