Lead (Pb2+) biosorption and bioaccumulation efficiency of Enterobacter chuandaensis DGI-2: Isotherm, kinetics and mechanistic study for bioremediation

Heavy metal (HM) contamination, particularly lead (Pb²⁺), threatens environmental and agricultural sustainability, necessitating effective remediation strategies. This study evaluates the Pb²⁺ sequestration potential of Enterobacter chuandaensis DGI-2, an HM-tolerant rhizobacterium isolated from a Pb-contaminated rhizosphere. DGI-2 exhibited high Pb²⁺ removal efficiency, achieving 94.73 % removal at 100 µg/mL and 69.09 % at 750 µg/mL over 96 h, primarily through cell surface and exopolysaccharide (EPS) adsorption. Biosorption studies demonstrated higher Pb²⁺ uptake in living biomass (102.95 mg/g, 68.63 %) than in dead biomass (98.61 mg/g, 65.74 %) under controlled conditions (0.5 g/L biomass, pH-6.5, 720 min). Mechanistic analyses revealed that Pb²⁺ adsorption primarily involved interactions with –OH, –COOH, and –PO₄³ ⁻ functional groups, facilitated by multilayer sorption, complexation, and ion exchange. Moreover, a 210.66 % increase in phosphatase activity promoted Pb²⁺ precipitation, forming stable Pb-phosphate minerals (e.g., Pb₅(PO₄)₃Cl, Pb₁₀(PO₄)₆(OH)₂), as confirmed by X-ray diffraction (XRD), significantly contributing to Pb sequestration. Regeneration studies demonstrated the biomass' reusability over four cycles. Soil microcosm experiments showed an 11.7–13.1 % reduction in bioavailable Pb, with greater stabilization in non-sterile soils, suggesting synergistic effects with native microbiota. Additionally, DGI-2 exhibited plant growth-promoting (PGP) traits, reducing phytotoxicity, enhancing soil health and phytostabilization potential, positioning it as a sustainable biosorbent for Pb²⁺ remediation.