香兰素在乙二醇诱导的高草酸大鼠模型中的抗尿石症活性

IF 2 2区 医学 Q2 UROLOGY & NEPHROLOGY
Aslam Khan, Sara Salim, Syed Muzzammil Masaud, Ashfaq Ahmad, Muhammad Furqan Akhtar, Safur Rehman Mandukhail
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引用次数: 0

摘要

香草素是一种从香草豆中提取的天然化合物,具有抗氧化和抗炎特性,可能有助于预防和治疗肾结石。因此,本研究旨在探讨香兰素对雄性高血氧Wistar大鼠的潜在抗尿石作用。通过计算分子对接研究,研究了相互作用过程,验证了香草醛在预防含草酸钙肾结石中的作用。利用软件工具分析药物配体的附加分子特征、吸收、分布、代谢和排泄(ADME)以及毒性。在饮用水中加入0.75%乙二醇(EG) 3周,在前3天加入1%氯化铵(AC)诱导大鼠尿结晶。分子对接分析显示,香兰素与人CTP:磷酸乙醇胺胞基转移酶(PDB ID: 3ELB)在C5P结合位点有较强的结合作用,结合亲和力为-7.6 kcal/mol,提示其抗尿石活性可能存在分子基础。体内研究表明,香兰素治疗剂量依赖(30、100和300 mg/kg体重)降低了高血氧大鼠的高血氧、高钙尿和肾脏晶体计数。我们目前的研究结果表明,香兰素具有潜在的抗尿石活性,对尿石症的治疗效果增强,可能是现代医学治疗尿石症的一种安全、有效和非侵入性的选择。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antiurolithic activity of vanillin in ethylene glycol-induced hyperoxaluric rat model.

Vanillin, a natural compound derived from vanilla beans, exhibits antioxidant and anti-inflammatory properties, which may contribute to the prevention and treatment of renal stones. Therefore, this study is aimed to investigate the potential antiurolithic effect of vanillin in male hyperoxaluric Wistar rats. Computational molecular docking studies were used to investigate the interaction process and verify vanillin's role in the prevention of kidney stones containing calcium oxalate. Software tools were utilized to analyze the drug ligands' additional molecular characteristics, absorption, distribution, metabolism, and excretion (ADME), and toxicity. Urinary crystals were induced in rats by adding 0.75% ethylene glycol (EG) in drinking water for 3 weeks, along with 1% ammonium chloride (AC) during the initial three days. Molecular docking analysis revealed strong binding interactions of vanillin with Human CTP: Phosphoethanolamine Cytidylyltransferase (PDB ID: 3ELB) at the C5P binding site, with a binding affinity of -7.6 kcal/mol, suggesting a potential molecular basis for its antiurolithic activity. In vivo study showed that vanillin treatment dose dependently (30, 100 and 300 mg/kg body weight) reduced hyperoxaluria, hypercalciuria and crystal counts in kidneys of hyperoxaluric rats. The current results of our study suggest that vanillin possesses potential antiurolithic activity, showing enhanced therapeutic effects in urolithiasis, which could be a safe, effective and non-invasive option in modern medicine for the management of urinary stones.

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来源期刊
Urolithiasis
Urolithiasis UROLOGY & NEPHROLOGY-
CiteScore
4.50
自引率
6.50%
发文量
74
期刊介绍: Official Journal of the International Urolithiasis Society The journal aims to publish original articles in the fields of clinical and experimental investigation only within the sphere of urolithiasis and its related areas of research. The journal covers all aspects of urolithiasis research including the diagnosis, epidemiology, pathogenesis, genetics, clinical biochemistry, open and non-invasive surgical intervention, nephrological investigation, chemistry and prophylaxis of the disorder. The Editor welcomes contributions on topics of interest to urologists, nephrologists, radiologists, clinical biochemists, epidemiologists, nutritionists, basic scientists and nurses working in that field. Contributions may be submitted as full-length articles or as rapid communications in the form of Letters to the Editor. Articles should be original and should contain important new findings from carefully conducted studies designed to produce statistically significant data. Please note that we no longer publish articles classified as Case Reports. Editorials and review articles may be published by invitation from the Editorial Board. All submissions are peer-reviewed. Through an electronic system for the submission and review of manuscripts, the Editor and Associate Editors aim to make publication accessible as quickly as possible to a large number of readers throughout the world.
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