肺炎克雷伯菌的o抗原多糖:抗原多样性的结构和分子基础。

IF 8 1区 生物学 Q1 MICROBIOLOGY
Chris Whitfield, Steven D Kelly, Tom D Stanton, Kelly L Wyres, Bradley R Clarke, Taylor J B Forrester, Agnieszka Kowalczyk
{"title":"肺炎克雷伯菌的o抗原多糖:抗原多样性的结构和分子基础。","authors":"Chris Whitfield, Steven D Kelly, Tom D Stanton, Kelly L Wyres, Bradley R Clarke, Taylor J B Forrester, Agnieszka Kowalczyk","doi":"10.1128/mmbr.00090-23","DOIUrl":null,"url":null,"abstract":"<p><p>SUMMARY<i>Klebsiella pneumoniae</i> is a gram-negative species, whose isolates are found in the environment and as commensals in the human gastrointestinal tract. This bacterium is among the leading causes of a range of nosocomial and community-acquired infections, particularly in immunocompromised individuals, where it can give rise to pneumonia, urinary tract infections, septicemia, and liver abscesses. Treatment of <i>K. pneumoniae</i> infections is compromised by the emergence of isolates producing carbapenemase and extended-spectrum β-lactamase enzymes, making it a high priority for new therapeutic approaches including vaccination and immunoprophylaxis. One potential target for these strategies is the O-antigen polysaccharide component of lipopolysaccharides, which are important virulence determinants for <i>K. pneumoniae</i>. Consideration of immunotherapeutic opportunities requires a comprehensive and fundamental understanding of O-polysaccharide structures, distribution of particular O serotypes in clinical isolates, and the potential for antigenic diversification. The number of recognized <i>K. pneumoniae</i> O-polysaccharide antigens has varied over time, complicated by the observation that some examples share similar structural (and potentially antigenically cross-reactive) elements, and by the existence of genetic loci for which corresponding O-polysaccharide structures have yet to be determined. Here, we provide a comprehensive integration of the current carbohydrate structures and genetic information, together with a proposal for an updated classification system for <i>K. pneumoniae</i> O-antigens, that is being implemented in Kaptive for molecular serotyping. The accumulated insight into O-polysaccharide assembly pathways is used to describe the molecular basis for O-antigen diversity in <i>K. pneumoniae</i>.</p>","PeriodicalId":18520,"journal":{"name":"Microbiology and Molecular Biology Reviews","volume":" ","pages":"e0009023"},"PeriodicalIF":8.0000,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"O-antigen polysaccharides in <i>Klebsiella pneumoniae</i>: structures and molecular basis for antigenic diversity.\",\"authors\":\"Chris Whitfield, Steven D Kelly, Tom D Stanton, Kelly L Wyres, Bradley R Clarke, Taylor J B Forrester, Agnieszka Kowalczyk\",\"doi\":\"10.1128/mmbr.00090-23\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>SUMMARY<i>Klebsiella pneumoniae</i> is a gram-negative species, whose isolates are found in the environment and as commensals in the human gastrointestinal tract. This bacterium is among the leading causes of a range of nosocomial and community-acquired infections, particularly in immunocompromised individuals, where it can give rise to pneumonia, urinary tract infections, septicemia, and liver abscesses. Treatment of <i>K. pneumoniae</i> infections is compromised by the emergence of isolates producing carbapenemase and extended-spectrum β-lactamase enzymes, making it a high priority for new therapeutic approaches including vaccination and immunoprophylaxis. One potential target for these strategies is the O-antigen polysaccharide component of lipopolysaccharides, which are important virulence determinants for <i>K. pneumoniae</i>. Consideration of immunotherapeutic opportunities requires a comprehensive and fundamental understanding of O-polysaccharide structures, distribution of particular O serotypes in clinical isolates, and the potential for antigenic diversification. The number of recognized <i>K. pneumoniae</i> O-polysaccharide antigens has varied over time, complicated by the observation that some examples share similar structural (and potentially antigenically cross-reactive) elements, and by the existence of genetic loci for which corresponding O-polysaccharide structures have yet to be determined. Here, we provide a comprehensive integration of the current carbohydrate structures and genetic information, together with a proposal for an updated classification system for <i>K. pneumoniae</i> O-antigens, that is being implemented in Kaptive for molecular serotyping. The accumulated insight into O-polysaccharide assembly pathways is used to describe the molecular basis for O-antigen diversity in <i>K. pneumoniae</i>.</p>\",\"PeriodicalId\":18520,\"journal\":{\"name\":\"Microbiology and Molecular Biology Reviews\",\"volume\":\" \",\"pages\":\"e0009023\"},\"PeriodicalIF\":8.0000,\"publicationDate\":\"2025-03-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microbiology and Molecular Biology Reviews\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1128/mmbr.00090-23\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbiology and Molecular Biology Reviews","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1128/mmbr.00090-23","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

摘要肺炎克雷伯菌是一种革兰氏阴性菌,其分离株存在于环境中,并作为共生体存在于人类胃肠道中。这种细菌是一系列医院和社区获得性感染的主要原因之一,特别是在免疫功能低下的个体中,它可引起肺炎、尿路感染、败血症和肝脓肿。肺炎克雷伯菌感染的治疗因产生碳青霉烯酶和广谱β-内酰胺酶的分离株的出现而受到损害,因此需要优先考虑新的治疗方法,包括疫苗接种和免疫预防。这些策略的一个潜在目标是脂多糖的o抗原多糖成分,这是肺炎克雷伯菌重要的毒力决定因素。考虑到免疫治疗的机会,需要对O-多糖结构、临床分离株中特定O血清型的分布以及抗原多样化的潜力有全面和基本的了解。已识别的肺炎克雷伯菌o -多糖抗原的数量随着时间的推移而变化,由于观察到一些例子具有相似的结构(和潜在的抗原性交叉反应)元件,以及存在尚未确定相应o -多糖结构的遗传位点,情况变得更加复杂。在这里,我们提供了当前碳水化合物结构和遗传信息的全面整合,并提出了一个更新的肺炎克雷伯菌o型抗原分类系统的建议,该系统正在Kaptive中实施分子血清分型。对o -多糖组装途径的积累见解用于描述肺炎克雷伯菌o -抗原多样性的分子基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
O-antigen polysaccharides in Klebsiella pneumoniae: structures and molecular basis for antigenic diversity.

SUMMARYKlebsiella pneumoniae is a gram-negative species, whose isolates are found in the environment and as commensals in the human gastrointestinal tract. This bacterium is among the leading causes of a range of nosocomial and community-acquired infections, particularly in immunocompromised individuals, where it can give rise to pneumonia, urinary tract infections, septicemia, and liver abscesses. Treatment of K. pneumoniae infections is compromised by the emergence of isolates producing carbapenemase and extended-spectrum β-lactamase enzymes, making it a high priority for new therapeutic approaches including vaccination and immunoprophylaxis. One potential target for these strategies is the O-antigen polysaccharide component of lipopolysaccharides, which are important virulence determinants for K. pneumoniae. Consideration of immunotherapeutic opportunities requires a comprehensive and fundamental understanding of O-polysaccharide structures, distribution of particular O serotypes in clinical isolates, and the potential for antigenic diversification. The number of recognized K. pneumoniae O-polysaccharide antigens has varied over time, complicated by the observation that some examples share similar structural (and potentially antigenically cross-reactive) elements, and by the existence of genetic loci for which corresponding O-polysaccharide structures have yet to be determined. Here, we provide a comprehensive integration of the current carbohydrate structures and genetic information, together with a proposal for an updated classification system for K. pneumoniae O-antigens, that is being implemented in Kaptive for molecular serotyping. The accumulated insight into O-polysaccharide assembly pathways is used to describe the molecular basis for O-antigen diversity in K. pneumoniae.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
18.80
自引率
0.80%
发文量
27
期刊介绍: Microbiology and Molecular Biology Reviews (MMBR), a journal that explores the significance and interrelationships of recent discoveries in various microbiology fields, publishes review articles that help both specialists and nonspecialists understand and apply the latest findings in their own research. MMBR covers a wide range of topics in microbiology, including microbial ecology, evolution, parasitology, biotechnology, and immunology. The journal caters to scientists with diverse interests in all areas of microbial science and encompasses viruses, bacteria, archaea, fungi, unicellular eukaryotes, and microbial parasites. MMBR primarily publishes authoritative and critical reviews that push the boundaries of knowledge, appealing to both specialists and generalists. The journal often includes descriptive figures and tables to enhance understanding. Indexed/Abstracted in various databases such as Agricola, BIOSIS Previews, CAB Abstracts, Cambridge Scientific Abstracts, Chemical Abstracts Service, Current Contents- Life Sciences, EMBASE, Food Science and Technology Abstracts, Illustrata, MEDLINE, Science Citation Index Expanded (Web of Science), Summon, and Scopus, among others.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信