原有免疫力对 SARS-CoV-2 变体对 CoronaVac 的体液和细胞反应的影响:聚焦常见人类冠状病毒。

IF 2.3 4区 医学 Q3 ALLERGY
Nawamin Pinpathomrat, Bunya Seeyankem, Ratchanon Sophonmanee, Jomkwan Ongarj, Smonrapat Surasombatpattana
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引用次数: 0

摘要

背景:由SARS-CoV-2引起的全球COVID-19大流行突出了了解疫苗引起的免疫反应的重要性。目的:本研究评估抗体和T细胞对灭活的冠状病毒疫苗的反应,以及对常见人类冠状病毒(hcov)的预先免疫在形成疫苗诱导免疫中的作用。方法:我们招募了64名参与者(男性17名,女性47名),在接种疫苗前后检测抗hcov的IgG水平。用野生型、Delta型和Omicron刺突肽刺激外周血单个核细胞(PBMCs),分析T细胞反应。结果:我们发现接种前存在HCoV-229E、HCoV-HKU1、HCoV-NL63和HCoV-OC43抗体。值得注意的是,接种疫苗后,预先存在的HCoV-229E和HCoV-HKU1抗体与抗rbd IgG水平呈正相关。接种前观察到野生型菌株已有CD4+ T细胞应答,δ再刺激后IFN-γ分泌显著减少,Omicron再刺激后部分恢复。与野生型相比,δ和Omicron再刺激后CD4+ T细胞产生的IL-4显著减少。与原始菌株相比,δ再刺激后CD8+ T细胞再次显示IL-4产生减少。结论:本研究表明,CoronaVac诱导了强大的体液和细胞免疫反应,尽管变异特异性反应各不相同。对某些hcov的预先免疫可能影响疫苗诱导的抗体反应,这强调了监测对新出现的SARS-CoV-2变体的免疫的重要性,并为未来的疫苗设计提供信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Impact of pre-existing immunity on humoral and cellular responses to CoronaVac in SARS-CoV-2 variants: A focus on common human Coronaviruses.

Background: The global COVID-19 pandemic, caused by SARS-CoV-2, has highlighted the importance of understanding immune responses elicited by vaccines.

Objective: This study evaluated antibody and T cell responses to the inactivated CoronaVac vaccine, as well as the role of pre-existing immunity to common human coronaviruses (HCoVs) in shaping vaccine-induced immunity.

Methods: We enrolled 64 participants (17 males and 47 females) and measured IgG levels against HCoVs before and after vaccination. T cell responses were analysed by stimulating peripheral blood mononuclear cells (PBMCs) with wild-type, Delta, and Omicron spike peptides.

Results: We found pre-existing antibodies against HCoV-229E, HCoV-HKU1, HCoV-NL63, and HCoV-OC43 were present before vaccination. Notably, a positive correlation was observed between pre-existing antibodies to HCoV-229E and HCoV-HKU1 and anti-RBD IgG levels post-vaccination. Pre-existing CD4+ T cell responses were observed for the wild-type strain before vaccination, with a significant reduction in IFN-γ secretion after Delta re-stimulation and partial restoration after Omicron re-stimulation. IL-4 production by CD4+ T cells was significantly reduced upon re-stimulation with Delta and Omicron compared to wild-type. CD8+ T cells again showed a reduction of IL-4 production after Delta re-stimulation compared to the original strain.

Conclusions: This work demonstrate that CoronaVac induces robust humoral and cellular immune responses, though variant-specific responses vary. Pre-existing immunity to certain HCoVs may influence vaccine-induced antibody responses, underscoring the importance of monitoring immunity to emerging SARS-CoV-2 variants and informing future vaccine design.

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来源期刊
CiteScore
12.80
自引率
0.00%
发文量
74
审稿时长
>12 weeks
期刊介绍: The Asian Pacific Journal of Allergy and Immunology (APJAI) is an online open access journal with the recent impact factor (2018) 1.747 APJAI published 4 times per annum (March, June, September, December). Four issues constitute one volume. APJAI publishes original research articles of basic science, clinical science and reviews on various aspects of allergy and immunology. This journal is an official journal of and published by the Allergy, Asthma and Immunology Association, Thailand. The scopes include mechanism, pathogenesis, host-pathogen interaction, host-environment interaction, allergic diseases, immune-mediated diseases, epidemiology, diagnosis, treatment and prevention, immunotherapy, and vaccine. All papers are published in English and are refereed to international standards.
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