Xiangjun Yang, Yi Hu, Haonan Zhou, Na Lu, Min Zhang, Zisheng Tang
{"title":"基于拟合物修饰过氧化物酶模拟纳米酶的粪肠球菌特异性捕获、检测和杀灭技术","authors":"Xiangjun Yang, Yi Hu, Haonan Zhou, Na Lu, Min Zhang, Zisheng Tang","doi":"10.1016/j.cej.2025.161848","DOIUrl":null,"url":null,"abstract":"Pathogenic bacteria in the human oral cavity are the leading causes of oral disease, like dental caries and root canal infections. Conventional broad-spectrum oral sterilization easily kills most bacteria indiscriminately, and may destroy microbial community balance and cause oral biofilm-related diseases. However, a platform that enables all-in-one specific capture, detecting, and killing for one type of bacteria rather than targeting other bacteria is desirable. Here, we demonstrate that a DNA aptamer-modified hierarchical magnetic copper silicate decorated Fe<sub>3</sub>O<sub>4</sub> nanozyme conjugate (Apt-CuSi@Fe) can efficiently mediate selective recognition, detection, and rapid killing of oral pathogen <em>Enterococcus faecalis</em> (<em>E. faecalis</em>). Needle-like magnetic nanostructures with peroxidase (POD)-like activity were synthesized by a simple hydrothermal method, and then DNA aptamers were functionalized on their surface to achieve magnetic targeted separation and visual detection of <em>E. faecalis</em>. Moreover, the efficient reactive oxygen species (ROS) generation ability and unique hierarchical structure of Apt-CuSi@Fe conjugates allowed for selective bacterial killing. At a concentration of 50 μg/mL, the sterilization efficiency of aptamer-functionalized nanozyme probes against <em>E. faecalis</em> exceeded 98 %, far superior to that of bare CuSi@Fe nanozymes (61.3 %). In addition, it effectively inhibited the formation of <em>E. faecalis</em> biofilms. The synergetic antibacterial mechanism combines physical damage to the bacterial membrane via piercing puncture with the efficient diffusion and binding of target-enhanced ROS, thereby promoting such a prompted antibacterial activity. The proposed all-in-one platform integrates precise capture, sensitive detection, and targeted killing of <em>E. faecalis</em>, with potential applications in the integrated diagnosis and treatment of oral infectious diseases and targeted eradication of bacterial infections.","PeriodicalId":270,"journal":{"name":"Chemical Engineering Journal","volume":"10 1","pages":""},"PeriodicalIF":13.2000,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Specific capture, detection, and killing of Enterococcus faecalis based on aptamer-modified peroxidase mimetic nanozymes\",\"authors\":\"Xiangjun Yang, Yi Hu, Haonan Zhou, Na Lu, Min Zhang, Zisheng Tang\",\"doi\":\"10.1016/j.cej.2025.161848\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Pathogenic bacteria in the human oral cavity are the leading causes of oral disease, like dental caries and root canal infections. Conventional broad-spectrum oral sterilization easily kills most bacteria indiscriminately, and may destroy microbial community balance and cause oral biofilm-related diseases. However, a platform that enables all-in-one specific capture, detecting, and killing for one type of bacteria rather than targeting other bacteria is desirable. Here, we demonstrate that a DNA aptamer-modified hierarchical magnetic copper silicate decorated Fe<sub>3</sub>O<sub>4</sub> nanozyme conjugate (Apt-CuSi@Fe) can efficiently mediate selective recognition, detection, and rapid killing of oral pathogen <em>Enterococcus faecalis</em> (<em>E. faecalis</em>). Needle-like magnetic nanostructures with peroxidase (POD)-like activity were synthesized by a simple hydrothermal method, and then DNA aptamers were functionalized on their surface to achieve magnetic targeted separation and visual detection of <em>E. faecalis</em>. Moreover, the efficient reactive oxygen species (ROS) generation ability and unique hierarchical structure of Apt-CuSi@Fe conjugates allowed for selective bacterial killing. At a concentration of 50 μg/mL, the sterilization efficiency of aptamer-functionalized nanozyme probes against <em>E. faecalis</em> exceeded 98 %, far superior to that of bare CuSi@Fe nanozymes (61.3 %). In addition, it effectively inhibited the formation of <em>E. faecalis</em> biofilms. The synergetic antibacterial mechanism combines physical damage to the bacterial membrane via piercing puncture with the efficient diffusion and binding of target-enhanced ROS, thereby promoting such a prompted antibacterial activity. The proposed all-in-one platform integrates precise capture, sensitive detection, and targeted killing of <em>E. faecalis</em>, with potential applications in the integrated diagnosis and treatment of oral infectious diseases and targeted eradication of bacterial infections.\",\"PeriodicalId\":270,\"journal\":{\"name\":\"Chemical Engineering Journal\",\"volume\":\"10 1\",\"pages\":\"\"},\"PeriodicalIF\":13.2000,\"publicationDate\":\"2025-03-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chemical Engineering Journal\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1016/j.cej.2025.161848\",\"RegionNum\":1,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, CHEMICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical Engineering Journal","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1016/j.cej.2025.161848","RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, CHEMICAL","Score":null,"Total":0}
Specific capture, detection, and killing of Enterococcus faecalis based on aptamer-modified peroxidase mimetic nanozymes
Pathogenic bacteria in the human oral cavity are the leading causes of oral disease, like dental caries and root canal infections. Conventional broad-spectrum oral sterilization easily kills most bacteria indiscriminately, and may destroy microbial community balance and cause oral biofilm-related diseases. However, a platform that enables all-in-one specific capture, detecting, and killing for one type of bacteria rather than targeting other bacteria is desirable. Here, we demonstrate that a DNA aptamer-modified hierarchical magnetic copper silicate decorated Fe3O4 nanozyme conjugate (Apt-CuSi@Fe) can efficiently mediate selective recognition, detection, and rapid killing of oral pathogen Enterococcus faecalis (E. faecalis). Needle-like magnetic nanostructures with peroxidase (POD)-like activity were synthesized by a simple hydrothermal method, and then DNA aptamers were functionalized on their surface to achieve magnetic targeted separation and visual detection of E. faecalis. Moreover, the efficient reactive oxygen species (ROS) generation ability and unique hierarchical structure of Apt-CuSi@Fe conjugates allowed for selective bacterial killing. At a concentration of 50 μg/mL, the sterilization efficiency of aptamer-functionalized nanozyme probes against E. faecalis exceeded 98 %, far superior to that of bare CuSi@Fe nanozymes (61.3 %). In addition, it effectively inhibited the formation of E. faecalis biofilms. The synergetic antibacterial mechanism combines physical damage to the bacterial membrane via piercing puncture with the efficient diffusion and binding of target-enhanced ROS, thereby promoting such a prompted antibacterial activity. The proposed all-in-one platform integrates precise capture, sensitive detection, and targeted killing of E. faecalis, with potential applications in the integrated diagnosis and treatment of oral infectious diseases and targeted eradication of bacterial infections.
期刊介绍:
The Chemical Engineering Journal is an international research journal that invites contributions of original and novel fundamental research. It aims to provide an international platform for presenting original fundamental research, interpretative reviews, and discussions on new developments in chemical engineering. The journal welcomes papers that describe novel theory and its practical application, as well as those that demonstrate the transfer of techniques from other disciplines. It also welcomes reports on carefully conducted experimental work that is soundly interpreted. The main focus of the journal is on original and rigorous research results that have broad significance. The Catalysis section within the Chemical Engineering Journal focuses specifically on Experimental and Theoretical studies in the fields of heterogeneous catalysis, molecular catalysis, and biocatalysis. These studies have industrial impact on various sectors such as chemicals, energy, materials, foods, healthcare, and environmental protection.