内镜下预用药对胃液微生物群影响的离体分析。

IF 1.7 Q3 GASTROENTEROLOGY & HEPATOLOGY

JGH Open Pub Date : 2025-03-20 DOI:10.1002/jgh3.70141

Toshiki Futakuchi, Hiroto Furuhashi, Kimio Isshi, Yuko Hara, Shingo Ono, Rina Kurokawa, Lena Takayasu, Wataru Suda, Kazuki Sumiyama

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引用次数: 0

摘要

背景与目的：二甲基硅氧烷（GAS）、利多卡因（XYL）和蛋白酶（PRO）是食管胃十二指肠镜检查（EGD）常用的前用药。然而，这些药物对胃微生物群的影响仍未被探索。因此，我们的目的是研究这些预用药对EGD患者胃液的影响。方法：内镜下抽取6例患者的胃液，分成6份进行体外分析。将样品与相应治疗组的预用药混合：GAS、XYL、PRO、MIX （GAS、XYL和PRO的混合物）和对照组(CTL1和2；没有药物治疗)。提取微生物DNA后，分析16S rRNA扩增子序列，从(1)基因组DNA （gDNA）数量、(2)α-多样性指数、Shannon指数、观察到的操作分类单位（OTUs）数量、Chao1指数、(3)加权和未加权UniFrac距离、(4)门、属的相对丰度等方面确定微生物区系分布。结果：6组间gDNA总提取量无显著差异。α-多样性指数各组间差异无统计学意义。尽管GAS、PRO和MIX在加权UniFrac距离上与技术重复有显著差异（p = 0.03），但在未加权UniFrac距离上没有观察到显著差异。然而，在门和属水平上观察到几种细菌微生物群的相对丰度存在显著差异。结论：预用药会影响特定门和属水平细菌群的微生物群。试验注册：大学医院医学信息网临床试验注册：UMIN-CTR 000040192和UMIN-CTR 000051289。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Ex Vivo Analysis of the Effect of Endoscopic Premedications on the Microbiota Profile in Gastric Juice

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Ex Vivo Analysis of the Effect of Endoscopic Premedications on the Microbiota Profile in Gastric Juice

Background and Aim

Dimethicone (GAS), lidocaine (XYL), and protease (PRO) are commonly used as premedications during esophagogastroduodenoscopy (EGD). However, the effects of these drugs on the gastric microbiota remain unexplored. Therefore, we aimed to investigate the effects of these premedications on gastric juice collected from patients undergoing EGD.

Methods

Gastric juice was endoscopically aspirated from six patients and divided into six aliquots for in vitro analysis. The samples were mixed with premedications in corresponding treatment sets: GAS, XYL, PRO, MIX (a mixture of GAS, XYL, and PRO), and control (CTL1 and 2; no medication treatment). After extraction of microbial DNA from the treated samples, the 16S rRNA amplicon sequence was analyzed to determine the microbiota profile in terms of (1) the amount of genomic DNA (gDNA), (2) α-diversity indices, Shannon index, number of observed operational taxonomic units (OTUs), and Chao1 index, (3) weighted and unweighted UniFrac distances, and (4) the relative abundance of phyla and genera.

Results

The total amount of extracted gDNA did not significantly differ between the six groups. The α-diversity indices did not significantly differ between treatment groups. Although GAS, PRO, and MIX differed significantly from the technical replicates in the weighted UniFrac distance (p = 0.03 all), no significant difference was observed in the unweighted UniFrac distance. However, significant differences were observed in the relative abundance of several bacterial microbiota at the phylum and genus levels.