[莱茵衣藻活细胞制剂缓解小麦幼苗镉胁迫的效果和机制]。

Q2 Environmental Science
Chun-Yan Lei, Ya-Nan Li, Meng-Jing Liang, Ze Yang, Yan Sun, Chun-Li Ji, Chun-Hui Zhang, Run-Zhi Li, Xi-Ping Sun, Hong-Li Cui
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引用次数: 0

摘要

镉污染不仅导致作物减产,而且通过食物链迁移和积累,对人类健康构成威胁。减少作物对Cd的吸收和富集,提高作物对Cd胁迫的抗性,是绿色健康农业可持续发展的重要课题。为了建立一种利用微藻控制Cd污染、提高作物抗逆性的新农艺技术,以莱茵衣藻和小麦为研究材料,对微藻阻断Cd迁移、提高作物抗Cd胁迫能力的作用和机制进行了系统研究。试验作物材料为冬小麦品种JM182和春小麦品种JC6。采用水培实验模拟50 mg·L-1 (Cd50)和100 mg·L-1 (Cd100)两种剂量的镉胁迫。采用常规的微藻培养方法,制备了D680 = 1.0 (A1.0)和D680 = 2.0 (A2.0)两种不同剂量的莱茵曲菌活细胞剂,并将其应用于Cd胁迫下的小麦幼苗。测定了两种镉胁迫下小麦幼苗的生长特征、光合作用、细胞抗氧化酶活性、镉吸收、转运和积累以及重金属转运相关基因转录等生理生化指标。结果表明,Cd胁迫对小麦幼苗生长具有重要的抑制作用。施用活细胞剂显著提高了青豆光合色素(叶绿素a、叶绿素b和类胡萝卜素)含量,激活了抗氧化酶系统(SOD、POD、CAT、GSH和APX)活性。微藻活细胞制剂还能降低镉胁迫对小麦幼苗生长的损害。此外,两种剂量(A1.0和A2.0)的莱茵假梭菌活细胞制剂均下调重金属吸收和运输相关基因(TaHMA2、TaHMA3、TaNramp1和TaLCT1)的表达。Cd含量分析表明,Cd胁迫导致了小麦幼苗Cd的积累,在两种Cd胁迫下,JC6的Cd含量均高于JM182。Cd50和Cd100胁迫导致JC6和JM182幼苗Cd含量分别高达185.01和342.11 mg·kg-1和176.76 mg·kg-1和317.65 mg·kg-1。值得注意的是,添加微藻活细胞剂显著降低了Cd胁迫下两个品种小麦幼苗的Cd富集。与Cd50胁迫下JC6小麦幼苗Cd水平相比,a2.0处理的JC6小麦幼苗根系和茎叶部位Cd积累水平分别降低了76.80%和66.91%,根系向茎叶部位Cd转运速率降低了27.58%。综上所述,微藻活细胞制剂可以通过下调重金属吸收和运输相关基因的表达,显著降低小麦幼苗对镉的吸收、积累和从根到茎叶器官的运输。微藻活细胞剂还能激活抗氧化系统,增加光合作用,从而减轻镉毒性,促进小麦幼苗生长发育。本研究结果为利用微藻活细胞制剂作为生物肥料或生物刺激剂来阻碍重金属污染物的迁移,提高植物对重金属胁迫的抗性提供了科学依据和新策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Effect and Mechanism of Chlamydomonas reinhartii Living Cell Agents in Alleviating Cd Stress on Wheat Seedlings].

Cadmium (Cd) pollution not only leads to the reduction in crop yields but also migrates and accumulates through the food chain, thus posing a threat to human health. It is an important issue to reduce Cd uptake and enrichment in crops and increase crop resistance to Cd stress for sustainable development of green-health agriculture. To establish a novel agronomic technique using microalgae to control Cd pollution and improve crop stress resistance, the microalga Chlamydomonas reinhardtii and wheat (Triticum aestivum)were employed for systematic investigation so as to the elucidate effects and mechanism of microalgae in blocking Cd migration and enhancing crop resistance to Cd stress. The test crop materials were the winter wheat variety JM182 and spring wheat variety JC6. Hydroponic experiments were used to simulate Cd stress at two dosages, 50 mg·L-1 (Cd50) and 100 mg·L-1 (Cd100). Two different dosages of C. reinhardtii living cell agents, i.e., the microalgal cell cultures with D680 = 1.0 (A1.0) and D680 = 2.0 (A2.0), were prepared using conventional microalgal-cultivation methods and subsequently used to treat wheat seedlings under Cd stress. Several physiological and biochemical parameters were determined for wheat seedlings under two doses of Cd stress, respectively, including growth features, photosynthesis, cellular antioxidant enzyme activity, Cd uptake, transportation and accumulation, and transcriptions of the genes associated with heavy metal transportation. The results showed that Cd stress crucially inhibited the growth of wheat seedlings. However, the application of C. reinhardtii living cell agents significantly increased the contents of photosynthetic pigments (chlorophyll a, chlorophyll b, and carotenoids) and activated the antioxidant enzyme system activities (SOD, POD, CAT, GSH, and APX). The microalgal living cell agents also reduced the damage of Cd stress on wheat seedling growth. Moreover, both doses (A1.0 and A2.0) of C. reinhardtii living cell agents downregulated the expression of the genes related to heavy metal absorption and transportation (TaHMA2, TaHMA3, TaNramp1,and TaLCT1). Analysis of Cd contents indicated that Cd stress resulted in Cd accumulation in wheat seedlings, with a higher level of Cd in JC6 than in JM182 under both doses of Cd stresses. Cd50 and Cd100 stresses led to Cd levels up to 185.01 mg·kg-1 and 342.11 mg·kg-1 in JC6 wheat seedlings and 176.76 mg·kg-1 and 317.65 mg·kg-1 in JM182, respectively. Notably, the addition of the microalgal living cell agent significantly reduced Cd enrichment in wheat seedlings of both varieties under Cd stresses. Compared to the Cd level in JC6 wheat seedlings under Cd50 stress, the Cd accumulation level in roots and stem-leaf parts of A2.0-treated JC6 wheat seedlings was reduced by 76.80% and 66.91%, respectively, followed by a 27.58% diminution of Cd transport rate from roots toward stem-leaf parts. Collectively, microalgal living cell agents could remarkably reduce Cd uptake, accumulation, and transportation from roots to stem-leaf organs of wheat seedlings by down-regulating gene expressions involved in heavy metal absorption and transportation. The microalgal living cell agent can also activate the antioxidant system and increase photosynthesis, thus mitigating Cd toxicity and promoting the growth and development of wheat seedlings. The present findings provide a scientific basis and new strategy for using microalgal living cell agents as bio-fertilizers or bio-stimulants to impede migration of heavy metal pollutants and enhance plant resistance to heavy metal stresses.

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来源期刊
环境科学
环境科学 Environmental Science-Environmental Science (all)
CiteScore
4.40
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15329
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