[南水北调中线泵站调节池表层沉积物中抗生素耐药基因的分布特征及影响因素]。

Q2 Environmental Science
Fei Li, Chun-Sheng Qiu, Chen-Chen Wang, Fei Xu, Dong Wang, Nan-Nan Liu, Xu Chen, Shao-Po Wang
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引用次数: 0

摘要

南水北调中线工程天津段原水泵站调节池是连接汉江原水与城市水厂的重要设施,是保障供水安全的重要设施。分别于2022年夏、秋、冬和2023年春采集调节池不同区域的地表沉积物样本。采用宏基因组测序技术分析了抗生素耐药基因(ARGs)的分布特征及影响因素,并分析了沉积物ARGs与理化指标、微生物群落结构、移动遗传元件(MGEs)的相关性。结果表明,调节池表层沉积物样品共检出20种抗生素耐药类型和921种ARGs亚型。不同采样时间和采样点样品的优势ARGs为多药耐药、MLS、四环素和糖肽,主要耐药机制为外排泵。相关性分析显示,沉积物的TN、NO3——N、TP和OM与前20个ARGs中的各种ARGs呈显著相关(P<0.05)。排名前20位的微生物属中,有19种与ARGs显著相关(P<0.05)。结合转移蛋白、重组酶和转座酶的MGEs类型与前20位ARGs呈显著正相关(P<0.05)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Distribution Characteristics and Influencing Factors of Antibiotic Resistance Genes in Surface Sediments of Regulating Pond in South-to-North Water Transfer Pumping Station].

As an important facility to ensure the safety of the water supply, the regulating pond of a raw water pumping station in the Tianjin Section of the middle route of the South-to-North Water Transfer Project linked the raw water from the Hanjiang River with the urban water plants. Surface sediment samples from different regions of the regulator pond were collected in summer, autumn, and winter of 2022 and spring of 2023, respectively. Metagenomic sequencing technology was used to analyze the distribution characteristics and influencing factors of antibiotic resistance genes (ARGs), and the correlation between sediment ARGs and physicochemical indices, microbial community structure, and mobile genetic elements (MGEs) was also analyzed. The results showed that 20 antibiotic resistance types and 921 ARGs subtypes were detected in the surface sediment samples of the regulator pond. The dominant ARGs in the samples at different sampling times and sampling points were multidrug resistance, MLS, tetracycline, and glycopeptides, and the main resistance mechanism was efflux pump. Correlation analysis showed that TN, NO3--N, TP, and OM of sediments were significantly correlated (P<0.05) with various ARGs of the top 20 ARGs. Among the top 20 microbial genera, 19 species were significantly correlated (P<0.05) with ARGs. The MGEs types of conjugate transfer protein, recombinase, and transposase were significantly positively correlated (P<0.05) with the top 20 ARGs.

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来源期刊
环境科学
环境科学 Environmental Science-Environmental Science (all)
CiteScore
4.40
自引率
0.00%
发文量
15329
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