小鼠臂旁外侧核向终纹床核和中央杏仁核的侧支投射。

IF 2.5 4区医学 Q3 NEUROSCIENCES

Neuroscience Letters Pub Date : 2025-03-18 DOI:10.1016/j.neulet.2025.138204

Li Zhang , Shuai Zhou , Yuen Fen Tan , Quan Fu Gan , Teoh Hoon Koon , Zhiqiang Wang , Shiqing Zhao , Yixuan Chen , Yi Sun , Pooi Pooi Leong

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引用次数: 0

摘要

背景：外侧臂旁核（LPBn）是处理和传递疼痛信号的关键中枢。方法：本研究通过立体定向注射AAV2/2Retro-hSyn-EGFP（简称EGFP）至小鼠终纹床核（BNST）和AAV2/2Retro-hSyn-tdTomato（简称tdTomato）至中央杏仁核（CeA），研究了LPBn的投射。随后，这些动物在AAV注射的对侧进行了幸免神经损伤（SNI）手术。为了检测降钙素基因相关肽（CGRP）和c-Fos的表达，我们进行了免疫荧光组织化学。结果：我们的研究结果表明，大约26% %的LPBn神经元被EGFP或tdTomato逆行标记为双标记。此外，绝大多数（85.49 %）双标记神经元为CGRP阳性（CGRP+）。在SNI小鼠中，几乎所有这些神经元（93.25 %）都是c-Fos阳性（c-Fos+），表明它们被激活。结论：这些发现表明，LPBn中的CGRP+神经元亚群通过轴突侧枝向BNST和CeA传递。值得注意的是，在SNI条件下，这些神经元可能在慢性疼痛的传递中发挥关键作用。

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Collateral projections from the lateral parabrachial nucleus to the bed nucleus of the stria terminalis and the central amygdala in mice

Background

The lateral parabrachial nucleus (LPBn) serves a critical hub for processing and transmitting pain signals. It has two main efferent pathways, the LPBn-CeA and LPBn-BNST, which are crucial for pain regulation and the management of negative emotions.

Methods

In our study, we investigated the projections from the LPBn by performing stereotaxic injections of AAV2/2Retro-hSyn-EGFP (abbreviated as EGFP) into the bed nucleus of the stria terminalis (BNST) and AAV2/2Retro-hSyn-tdTomato (abbreviated as tdTomato) into the central amygdala (CeA) in mice. The animals subsequently underwent spared nerve injury (SNI) surgery on the contralateral side to the AAV injections. To examine the expression of calcitonin gene-related peptide (CGRP) and c-Fos, we conducted immunofluorescent histochemistry.

Results

Our results indicated that approximately 26 % of the LPBn neurons retrogradely labeled with either EGFP or tdTomato were dual-labeled with both markers. Moreover, a significant majority (85.49 %) of these double-labeled neurons were CGRP positive (CGRP⁺). In mice subjected to SNI, nearly all of these neurons (93.25 %) were c-Fos positive (c-Fos⁺), indicating that they were activated.

Conclusion

These findings suggest that a subset of CGRP⁺ neurons in the LPBn projects to both the BNST and CeA via axon collaterals. Notably, under SNI conditions, these neurons may play a critical role in the transmission of chronic pain.

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来源期刊

Neuroscience Letters 医学-神经科学

CiteScore

5.20

自引率

0.00%

发文量

408

审稿时长

50 days

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